Mechanisms of transport through the Golgi complex
Mechanisms of transport through the Golgi complex
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DOI：
10.1242/jcs.032581
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发表时间：
2009-02-15
影响因子：
4
通讯作者：
Jackson, Catherine L.
中科院分区：
文献类型：
--
作者：
Jackson, Catherine L.
高尔基复合体是分泌途径的中央分选和加工站，确保在内质网中合成的货物蛋白被适当地糖基化并包装到载体中以运输到其最终目的地。最近的两项研究强调了这样一个事实，即膜脂的性质在高尔基体的结构组织和运输中起着关键作用。安东尼实验室已经证明了一种机制，通过这种机制，一端含有膜曲率传感域的高尔基体系绳可以以可逆的方式将高度弯曲和平坦的膜连接在一起。通过这种方式，以定向方式将膜结合在一起的强相互作用可以容易地随着膜的性质改变而被破坏。Lippincott-Schwartz实验室开发了一种新的高尔基体内运输模型，称为快速分配模型，该模型将脂质运输作为一个组成部分。模拟结果表明，将脂质分选到连接到每个高尔基体池的加工和出口结构域中，以及在整个高尔基体中双向运输以允许蛋白质与其优选的脂质环境相关联，足以驱动蛋白质通过分泌途径转运。虽然只是一个原则上的证明，这个模型第一次调用的驱动力内高尔基体贩运的脂质分选，并为未来的实验工作提供了一个框架。
The Golgi complex is the central sorting and processing station of the secretory pathway, ensuring that cargo proteins, which are synthesized in the endoplasmic reticulum, are properly glycosylated and packaged into carriers for transport to their final destinations. Two recent studies highlight the fact that properties of membrane lipids play key roles in Golgi structural organization and trafficking. The Antonny laboratory has demonstrated the mechanism by which a Golgi tether containing a membrane-curvature-sensing domain at one end can link highly curved and flat membranes together in a reversible manner. In this way, a strong interaction that binds membranes together in an oriented fashion can easily be disrupted as the properties of the membranes change. The Lippincott-Schwartz laboratory has developed a new model for intra-Golgi trafficking, called the rapid-partitioning model, which incorporates lipid trafficking as an integral part. Simulations reveal that the sorting of lipids into processing and export domains that are connected to each Golgi cisterna, and bidirectional trafficking throughout the Golgi to allow proteins to associate with their preferred lipid environment, is sufficient to drive protein transport through the secretory pathway. Although only a proof in principle, this model for the first time invokes lipid sorting as the driving force in intra-Golgi trafficking, and provides a framework for future experimental work.