Differential and dynamic regulation of miR398 in response to ABA and salt stress in Populus tremula and Arabidopsis thaliana
Differential and dynamic regulation of miR398 in response to ABA and salt stress in Populus tremula and Arabidopsis thaliana
复制标题
DOI：
10.1007/s11103-009-9508-8
复制
发表时间：
2009-09-01
影响因子：
5.1
通讯作者：
Tang, Guiliang
中科院分区：
文献类型：
--
作者：
Jia, Xiaoyun;Wang, Wang-Xia;Tang, Guiliang
MicroRNAs(MiRNAs)是一类类似于22个核苷酸(NT)的内源性小RNA，在植物和动物转录后水平下调基因表达方面起着关键作用。各种研究已经确定了许多在压力处理下上调或下调的miRNAs。在此，我们试图了解不同植物在脱落酸(ABA)和盐(NaC L)胁迫下miRNAs的时间调控。我们的结果表明，miR398对ABA和盐胁迫的响应在植物中的调节比以前报道的更动态。在杨树中，miR398首先在ABA或盐胁迫3-4h后诱导产生。然而，这种诱导在48小时后下降，并在长时间的应激(72小时)后再次积累。我们把这种监管称为动态监管。相反，在盐胁迫下miR398的这种动态调节在拟南芥中是完全不存在的，在拟南芥中miR398被稳定地单向抑制。有趣的是，ABA处理引起了拟南芥miR398的偏离动态调节，表现出与杨树相反的反应。我们将拟南芥和杨树之间的调控差异称为差异调控。此外，miR398靶标铜超氧化物歧化酶1(CSD1)的表达与miR398水平呈负相关，表明在非生物胁迫下，miR398主要控制这一特定靶标的表达。综上所述，这些数据一致地表明了ABA和盐胁迫对miR398及其代表靶标CSD1之间的相关性调控，并提出了植物中miRNAs的调控是双重的可能性：植物物种内的动态调控和不同植物物种之间的差异调控。
MicroRNAs (miRNAs) are endogenous small RNAs of similar to 22 nucleotides (nt) that play a key role in down regulation of gene expression at the post-transcriptional level in plants and animals. Various studies have identified numerous miRNAs that were either up regulated or down regulated upon stress treatment. Here, we sought to understand the temporal regulation of miRNAs in different plant species under abscisic acid (ABA) and salt (NaCl) stress. Our results showed that the regulation of miR398 in response to ABA and salt stress was more dynamic in plants than previously reported. In poplars, miR398 was first induced upon 3-4 h of ABA or salt stress. However, this induction declined after 48 h and finally accumulated again over a prolonged stress (72 h). We referred to this kind of regulation as dynamic regulation. In contrast, such dynamic regulation of miR398 under salt stress was completely absent in Arabidopsis, in which miR398 was steadily and unidirectionally suppressed. Interestingly, ABA treatment caused a deviate dynamic regulation of miR398 in Arabidopsis, showing an opposite response as compared to that in poplars. We referred to the difference in regulation between Arabidopsis and poplars as differential regulation. Furthermore, the expression of the miR398 target, copper superoxide dismutase1 (CSD1), was in reverse correlation with the miR398 level, suggesting a control of this specific target expression predominantly by miR398 under abiotic stress. Together, these data consistently show a correlated regulation between miR398 and its representative target, CSD1, by ABA and salt stresses, and raise the possibility that regulation of miRNAs in plants is twofold: a dynamic regulation within a plant species and a differential regulation between different plant species.