Synthesis of biodegradable polycationic methoxy poly(ethylene glycol)-polyethylenimine-chitosan and its potential as gene carrier
Synthesis of biodegradable polycationic methoxy poly(ethylene glycol)-polyethylenimine-chitosan and its potential as gene carrier
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可生物降解的聚阳离子甲氧基聚乙二醇-聚乙烯亚胺-壳聚糖的合成及其作为基因载体的潜力
DOI：
10.1016/j.carbpol.2009.04.019
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发表时间：
2009-08-04
影响因子：
11.2
通讯作者：
Gao, Rong
中科院分区：
文献类型：
--
作者：
Xu, Zhigang;Wan, Xiaoping;Gao, Rong
Methoxy poly(ethylene glycol)-polyethylenimine-chitosan (mPEG-PEI-CS) was synthesized via chitosan conjugated with polyethylenimine and methoxy poly(ethylene glycol). The intermediates and final copolymer were characterized and confirmed by H-1 NMR and FT-IR spectra. mPEG-PEI-CS was water soluble and its intrinsic viscosity was 0.446 dL/g. The contents of mPEG and PEI conjugated in the copolymer were 51.3% (w/w) and 28.9% (w/w), and the degree of substitution of PEI by mPEG was 176%. Gel electrophoresis confirmed that DNA was retained completely by the copolymer nanoparticles. The average diameter and zeta potential of mPEG-PEI-CS/DNA were 155 nm and 17.5 mV. The transfection of human embryonic kidney 293 (HEK293) cells proved that mPEG-PEI-CS/VRfat-1 plasmid had little toxicity on the growth and gene expression of cells, and the ratio of omega-3/omega-6 fatty acids was obviously increased after 72 h transfection compared to CS/VRfat-1 (P < 0.05). These indicated that mPEG-PEI-CS was a promising effective gene delivery and package molecule. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
