The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules
The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules
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DOI：
10.1016/s1074-7613(00)80425-2
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发表时间：
1997-02-01
期刊：
影响因子：
32.4
通讯作者：
Kronenberg, M
中科院分区：
文献类型：
--
作者：
Prigozy, TI;Sieling, PA;Kronenberg, M
我们的特点是CD1b介导的介绍途径的分枝杆菌脂聚糖脂阿拉伯甘露聚糖（LAM）在单核细胞衍生的抗原呈递细胞。巨噬细胞甘露糖受体（MR）负责摄取LAM。MR功能的拮抗抑制LAM的内化和该抗原向LAM反应性T细胞的呈递。细胞内MR在早期内体中最丰富，但它们也位于MHC II类抗原加载（MIIC）的隔室中。内化的LAM被转运到晚期内体、溶酶体和MIIC。MR与CD1b分子共定位，表明MR可以将LAM递送到晚期内体以加载到CD1b上。LAM酸CD1b共定位于细胞器中，可能是脂聚糖抗原加载的位点。该途径将先天免疫系统的受体对微生物抗原的识别与适应性T细胞应答的诱导联系起来。
We have characterized the CD1b-mediated presentation pathway for the mycobacterial lipoglycan lipoarabinomannan (LAM) in monocyte-derived antigen-presenting cells. The macrophage mannose receptor (MR) was responsible for uptake of LAM. Antagonism of MR function inhibited both the internalization of LAM and the presentation of this antigen to LAM-reactive T cells. Intracellular MRs were most abundant in early endosomes, but they also were located in the compartment for MHC class II antigen loading (MIIC). Internalized LAM was transported to late endosomes, lysosomes, and MIICs. MRs colocalized with CD1b molecules, suggesting that the MR could deliver LAM to late endosomes for loading onto CD1b. LAM acid CD1b colocalized in organelles that may be sites of lipoglycan antigen loading. This pathway links recognition of microbial antigens by a receptor of the innate immune system to the induction of adaptive T cell responses.