Metabolic engineering in methanotrophic bacteria
Metabolic engineering in methanotrophic bacteria
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DOI：
10.1016/j.ymben.2015.03.010
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发表时间：
2015-05-01
影响因子：
8.4
通讯作者：
Lidstrom, Mary E.
中科院分区：
文献类型：
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作者：
Kalyuzhnaya, Marina G.;Puri, Aaron W.;Lidstrom, Mary E.
甲烷，如天然气或沼气，是（生物）化学合成中最便宜的碳源。将这种简单烷烃可规模化地生物升级为化学品和燃料可以为许多具有大环境足迹的行业带来新的可持续解决方案，例如天然气/石油生产，垃圾填埋场，废水处理和畜牧业。以甲烷为原料的微生物生物催化已经断断续续地进行了将近半个世纪，但几乎没有持久的成功。今天，生物工程和系统生物学为代谢系统调节提供了新的机会，并为基于甲烷的生物工业的概念带来了新的乐观。本文综述了微生物甲烷利用代谢工程的最新进展。一些想法有关生产乙酰辅酶A和丙酮酸，生物转化的两个主要前体的代谢改进，提出。我们还讨论了目前有氧甲烷利用知识的主要差距，必须解决，以释放甲烷为基础的生物系统的全部潜力。(C)2015年国际代谢工程学会。爱思唯尔公司出版All rights reserved.
Methane, as natural gas or biogas, is the least expensive source of carbon for (bio)chemical synthesis. Scalable biological upgrading of this simple alkane to chemicals and fuels can bring new sustainable solutions to a number of industries with large environmental footprints, such as natural gas/petroleum production, landfills, wastewater treatment, and livestock. Microbial biocatalysis with methane as a feedstock has been pursued off and on for almost a half century, with little enduring success. Today, biological engineering and systems biology provide new opportunities for metabolic system modulation and give new optimism to the concept of a methane-based bio-industry. Here we present an overview of the most recent advances pertaining to metabolic engineering of microbial methane utilization. Some ideas concerning metabolic improvements for production of acetyl-CoA and pyruvate, two main precursors for bioconversion, are presented. We also discuss main gaps in the current knowledge of aerobic methane utilization, which must be solved in order to release the full potential of methane-based biosystems. (C) 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.