Determination of diethylstilbestrol by enhancement of luminol–hydrogen peroxide–tetrasulfonated cobalt phthalocyanine chemiluminescence
Determination of diethylstilbestrol by enhancement of luminol–hydrogen peroxide–tetrasulfonated cobalt phthalocyanine chemiluminescence
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DOI：
10.1016/j.aca.2003.12.003
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发表时间：
2004-04
影响因子：
6.2
通讯作者：
Jian Wang;Huazhen Ye;Zhou Jiang;N. Chen;Jin-ling Huang
中科院分区：
文献类型：
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作者：
Jian Wang;Huazhen Ye;Zhou Jiang;N. Chen;Jin-ling Huang
在碱性介质中，己烯雌酚对四磺酸酞菁钴催化鲁米诺-H2 O2化学发光反应有增敏作用，据此建立了流动注射化学发光法测定己烯雌酚的新方法。在最佳条件下，己烯雌酚的线性范围为1.00×10− 7 ~ 4.00×10− 6 mol/l。该方法灵敏度高（检出限为6.42×10− 8 mol/l，S/N=3），重复性好（相对标准偏差为2.6%，n=11），准确度高（回收率达92%）。该方法已成功地应用于药物制剂中己烯雌酚的测定。双烯雌酚和己雌酚是与己烯雌酚相似的烷基酚类化合物，可以用该方法检测。此外，本文还对类雌激素化合物的增效机制进行了探讨。
The ability of diethylstilbestrol to enhance the chemiluminescence reaction of luminol–H2O2, catalyzed by tetrasulfonated cobalt phthalocyanine in an alkaline medium, has been exploited to develop a new flow injection chemiluminescence method for the determination of diethylstilbestrol. Under the optimum conditions, the linearity of the calibration graph for the determination of diethylstilbestrol was in the range from 1.00×10−7to 4.00×10−6mol/l. The assay was sensitive (the detection limit was 6.42×10−8mol/l, S/N=3), reproducible (the relative standard deviation was 2.6%, n=11), and accurate (recovery up to 92%). The method has been successfully applied to the determination of diethylstilbestrol in the pharmaceutical formulations. Dienestrol and hexestrol were specifically alkylphenols, which are similar to diethylstilbestrol, could be detected by this method. Furthermore, the enhanced mechanism of estrogen-like compounds was also discussed in this paper.