T Cell Activation State in the Induced Sputum of Asthmatics Treated with Budesonide
T Cell Activation State in the Induced Sputum of Asthmatics Treated with Budesonide
复制标题
布地奈德治疗哮喘患者诱导痰中T细胞活化状态
DOI：
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发表时间：
2010
影响因子：
3.5
通讯作者：
C. Calabrese
中科院分区：
文献类型：
--
作者：
A. Vatrella;F. Perna;G. Pelaia;R. Parrella;R. Maselli;S. Marsico;C. Calabrese
支气管高反应性和嗜酸性粒细胞和T淋巴细胞的气道浸润是哮喘的关键特征。特别是，目前认为CD 4 + T细胞作为哮喘炎症反应的启动者和协调者发挥关键作用，因此，它们代表皮质类固醇治疗的关键靶点。因此，本研究的目的是评估轻度哮喘患者吸入皮质类固醇治疗对以下参数的影响：（i）CD 4 + T细胞的功能状态;（ii）气道嗜酸性粒细胞增多症;（iii）支气管对乙酰甲胆碱的高反应性。研究由20名哮喘、特应性受试者完成，分为两组，每组10人，分别接受吸入性布地奈德（200 μg，每日两次）或特布他林（500 μg，每日两次）治疗12周。通过流式细胞术在基线和治疗1、4、8和12周后测量诱导痰中CD 4 + T细胞活化标志物的表达，结果显示与对照组相比，布地奈德组HLA-DR和CD 25表面蛋白下调;这些差异在4-12周内具有统计学显著性。布地奈德还诱导诱导痰中可检测到的嗜酸性粒细胞百分比快速急剧下降，以及对乙酰甲胆碱的气道高反应性的更渐进的进行性改善。因此，除了评估支气管炎症的各种指标，流式细胞术可以可靠地应用于诱导痰，以监测，即使在轻度症状的患者，抗哮喘治疗对T细胞活化的影响。
Bronchial hyperresponsiveness and airway infiltration with eosinophils and T lymphocytes are key features of asthma. In particular, CD4+ T cells are currently believed to play a pivotal role as initiators and coordinators of the asthmatic inflammatory response and, therefore, they represent a crucial target of corticosteroid treatment. The aim of the present investigation is thus to evaluate, in patients with mild asthma, the effects of inhaled corticosteroid therapy on the following parameters: (i) functional state of CD4+ T cells; (ii) airway eosinophilia; (iii) bronchial hyperresponsiveness to methacholine. The study was completed by twenty asthmatic, atopic subjects, subdivided into two groups of ten and treated for 12 weeks with either inhaled budesonide (200 μg twice daily) or terbutaline alone (500 μg twice daily), respectively. Expression of CD4+ T cell activation markers was measured in induced sputum at baseline and after 1, 4, 8 and 12 weeks of treatment by flow cytometry, which showed a down-regulation of HLA-DR and CD25 surface proteins in the budesonide group, compared with the control group; these differences resulted as being statistically significant through weeks 4–12. Budesonide also induced a quick, sharp reduction in the percentage of eosinophils detectable in induced sputum, as well as a more gradual progressive improvement in airway hyperresponsiveness to methacholine. Therefore, in addition to assessing various indices of bronchial inflammation, flow cytometry can be reliably applied to induced sputum in order to monitor, even in mildly symptomatic patients, the effects of anti-asthma treatments on T cell activation.
影响因子：
15.9
作者：
Ray, A;Cohn, L
通讯作者：
Cohn, L
DOI：
10.1164/rccm.200903-0392oc
发表时间：
2009-09-01
影响因子：
24.7
作者：
Woodruff, Prescott G.;Modrek, Barmak;Fahy, John V.
通讯作者：
Fahy, John V.