T1 Mapping on Gd-EOB-DTPA-Enhanced MRI for the Prediction of Oxaliplatin-Induced Liver Injury in a Mouse Model
T1 Mapping on Gd-EOB-DTPA-Enhanced MRI for the Prediction of Oxaliplatin-Induced Liver Injury in a Mouse Model
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Gd-EOB-DTPA 增强 MRI 上的 T1 映射可预测小鼠模型中奥沙利铂引起的肝损伤
DOI：
10.1002/jmri.27377
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发表时间：
2020
影响因子：
4.4
通讯作者：
Mengsu Zeng
中科院分区：
文献类型：
--
作者：
Li Yang;Ying Ding;Shengxiang Rao;Caizhong Chen;Mengsu Zeng
背景奥沙利铂引起的肝损伤（OILI）不仅损害肝再生，而且增加术后发病率和死亡率。因此，非侵入性、准确和早期诊断 OILI 是强制性的。目的评估乙氧基苄基二亚乙基三胺五乙酸钆 (Gd-EOB-DTPA) 增强 MRI 上 T1 映射用于评估小鼠模型中 OILI 的潜力。研究类型案例对照，动物模型。动物模型包括 30 只奥沙利铂治疗小鼠和 10 只对照小鼠。场强体积插值屏气检查序列：带有相控阵动物 8 通道线圈的 3T 扫描仪。在注射 Gd-EOB-DTPA 之前和之后的肝胆期 (HBP) 进行 T1 绘图。评估 测量肝实质的 T1 弛豫时间并计算减少率 (ΔT1%)。组织学结果用作标准参考。统计测试采用 Kruskal-Wallis 检验和使用 Mann-WhitneyU 检验进行成对比较来比较各组的参数。进行了 Spearman 等级相关检验和受试者工作特征 (ROC) 分析。使用DeLong方法比较曲线下面积（AUC）。结果组织学上，将小鼠分为正常（n = 10）、肝细胞变性无纤维化（n = 16）和肝细胞变性伴纤维化（n = 14）。 HBP T1松弛时间随着OILI的严重程度而增加（rho = 0.60，P< 0.05），并且ΔT1%随着OILI的严重程度而减少（rho = –0.78，P< 0.05）。 ΔT1%区分无纤维化肝细胞变性与正常肝脏的AUC为0.92，但HBP T1松弛时间无法区分它们（P=0.09）。在区分有纤维化的 OILI 与正常肝脏和无纤维化的 OILI 时，HBP T1 松弛时间的 AUC 分别为 0.96 和 0.95，ΔT1% 的 AUC 分别为 0.96 和 0.95。 数据结论 Gd-EOB-DTPA 增强 MRI 的 HBP T1 松弛时间和 ΔT1% 对于评估 OILI 是有用的。 ΔT1%在检测早期肝损伤方面可能比HBP T1弛豫时间更敏感。证据级别2。技术功效阶段5。
BackgroundOxaliplatin‐induced liver injury (OILI) not only impairs hepatic regeneration but also increases postoperative morbidity and mortality. Therefore, noninvasive, accurate, and early diagnosis of OILI is mandatory.PurposeTo evaluate the potential of T1mapping on gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd‐EOB‐DTPA)‐enhanced MRI for assessing OILI in a mouse model.Study TypeCase control, animal model.Animal ModelThirty oxaliplatin‐treated mice and 10 control mice were included.Field StrengthVolumetric interpolated breath‐hold examination sequence: 3T scanner with a phased‐array animal 8‐channel coil. T1mapping before and at hepatobiliary phase (HBP) after injection of Gd‐EOB‐DTPA were undertaken.AssessmentT1relaxation times of the liver parenchyma were measured and the reduction rate (ΔT1%) was calculated. Histological findings were used as a standard reference.Statistical TestsThe Kruskal–Wallis test with pairwise comparisons using the Mann–WhitneyU‐test were applied to compare the parameters across groups. Spearman's rank correlation test and receiver operating characteristics (ROC) analyses were performed. Areas under the curves (AUCs) were compared using the DeLong method.ResultsHistologically, mice were classified as normal (n =10), hepatocellular degeneration without fibrosis (n =16), and hepatocellular degeneration with fibrosis (n =14). HBP T1relaxation time increased with the severity of OILI (rho = 0.60,P< 0.05), and ΔT1% decreased with the severity of OILI (rho = –0.78,P< 0.05). AUC was 0.92 for ΔT1% in differentiating hepatocellular degeneration without fibrosis from normal liver, but HBP T1relaxation time could not distinguish them (P= 0.09). AUCs were 0.96 and 0.95 for HBP T1relaxation time, and 0.90 and 0.84 for ΔT1% in discriminating OILI with fibrosis from normal liver and OILI without fibrosis.Data ConclusionHBP T1relaxation time and ΔT1% of Gd‐EOB‐DTPA enhanced MRI was useful for assessing OILI. ΔT1% may be more sensitive than HBP T1relaxation time in detecting early stage of liver injury.Level of Evidence2.Technical Efficacy Stage5.