Poly(aspartic acid)-based Degradable Assemblies for Highly Efficient Gene Delivery
Poly(aspartic acid)-based Degradable Assemblies for Highly Efficient Gene Delivery
复制标题
用于高效基因传递的聚天冬氨酸可降解组件
DOI：
10.1021/am506730t
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发表时间：
2015
影响因子：
9.5
通讯作者：
Fujian Xu
中科院分区：
文献类型：
--
作者：
Jingjun Nie;Xuebo Dou;Hao Hu;Bingran Yu;DaFu Chen;Renxian Wang;Fujian Xu
聚天冬氨酸(PAsp)具有细胞毒性低、可降解性好、生物相容性好等特点，是开发新型给药系统的理想材料。在这项工作中，通过以环糊精(CD)为核心的PAsp聚阳离子与含苯基的PAsp骨架之间的主客体相互作用，制备了一系列新的基于PAsp的可降解超分子组装体，用于有效的基因治疗。这种超分子组装体具有良好的降解性、增强的PDNA缩合能力和低的细胞毒性。更重要的是，在不同的N/P比下，超分子组件的基因转染率远高于以CD为核心的PAsp为基础的组件。此外，通过自杀基因治疗系统验证了组装体的有效抗肿瘤能力。本研究将为制备可降解的超分子给药系统提供一种新的途径。
Due to its good properties such as low cytotoxicity, degradability, and biocompatibility, poly(aspartic acid) (PAsp) is a good candidate for the development of new drug delivery systems. In this work, a series of new PAsp-based degradable supramolecular assemblies were prepared for effective gene therapy via the host–guest interactions between the cyclodextrin (CD)-cored PAsp-based polycations and the pendant benzene group-containing PAsp backbones. Such supramolecular assemblies exhibited good degradability, enhanced pDNA condensation ability, and low cytotoxicity. More importantly, the gene transfection efficiencies of supramolecular assemblies were much higher than those of CD-cored PAsp-based counterparts at various N/P ratios. In addition, the effective antitumor ability of assemblies was demonstrated with a suicide gene therapy system. The present study would provide a new means to produce degradable supramolecular drug delivery systems.