Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-finger protein, antisense to the RAF1 proto-oncogene
Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-finger protein, antisense to the RAF1 proto-oncogene
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DOI：
10.1006/geno.2001.6627
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发表时间：
2001-10-01
期刊：
影响因子：
4.4
通讯作者：
Nicholls, RD
中科院分区：
文献类型：
--
作者：
Gray, TA;Azama, K;Nicholls, RD
天然内源性反义RNA已在多个基因座中报道，在某些情况下有证据支持反义转录物的调节作用。在这里，我们描述了一种新的基因，makorin环锌指-2（MKRN 2），在哺乳动物中的基因RAF 1的重叠和反义。RAF 1同源基因3'非翻译区的系统发育分析表明，这种关系可能已经存在了4.5亿年。我们还在两种鱼类中鉴定了MKRN 2直系同源物。这使得在脊椎动物进化的早期，超过4.5亿年前，从祖先MKRN 1基因中产生这个位点的基因复制事件。北方印迹分析显示，人MKRN 2和RAF 1在组织和细胞系中共表达，提高了mRNA双链体形成的可能性。编码的makorin-2蛋白可能是一种功能未知的核糖核蛋白，但其保守性表明其具有重要的细胞作用。这里提供的数据描述了一个保守的脊椎动物MKRN 2基因，与RAF 1基因座密切相关。
Natural endogenous antisense RNAs have been reported in multiple loci, with evidence in some cases supporting a regulatory role for the antisense transcript. Here, we describe a novel gene, makorin RING zinc finger-2 (MKRN2), that overlaps and is antisense to the gene RAF1 in mammals. Phylogenetic analysis of the 3' untranslated region of RAF1 orthologues suggests that this relationship may have existed for up to 450 million years. We have also identified MKRN2 orthologues in two species of fish. This places the gene duplication event that created this locus from an ancestral MKRN1 gene early in vertebrate evolution, over 450 million years ago. Northern blot analyses show that human MKRN2 and RAF1 are co-expressed in tissues and cell lines, raising the possibility of mRNA duplex formation. The encoded makorin-2 protein is likely a ribonucleoprotein of unknown function, but its conservation suggests an important cellular role. The data presented here describe a conserved vertebrate MKRN2 gene that is closely associated with the RAF1 locus.