Engineered biosynthesis of plant polyketides: Manipulation of chalcone synthase
Engineered biosynthesis of plant polyketides: Manipulation of chalcone synthase
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DOI：
10.1021/ol052912h
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发表时间：
2006-02-02
期刊：
影响因子：
5.2
通讯作者：
Noguchi, H
中科院分区：
文献类型：
--
作者：
Abe, I;Watanabe, T;Noguchi, H
查尔酮合成酶(CHS)是一种植物特有的III型聚酮合成酶，催化4-香豆酰辅酶A与3个丙二酰辅酶A的缩合反应。令人惊讶的是，黄芩S338V突变体CHS从8个丙二酰辅酶A分子中产生了八肽SEK4/SEK4b。此外，在一个CHS三重突变体(T197G/G256L/S338T)中，八肽的形成活性显著提高。官能团的转化是基于活性中心腔内化学惰性残基的简单空间调节。
[GRAPHICS]Chalcone synthase (CHS) is a plant-specific type III polyketide synthase catalyzing condensation of 4-coumaroyl-CoA with three molecules of malonyl-CoA. Surprisingly, it was demonstrated that S338V mutant of Scutellaria baicalensis CHS produced octaketides SEK4/SEK4b from eight molecules of malonyl-CoA. Further, the octaketides-forming activity was dramatically increased in a CHS triple mutant (T197G/G256L/S338T). The functional conversion is based on the simple steric modulation of a chemically inert residue lining the active-site cavity.