Molecular cloning and characterization of rice 6-phosphogluconate dehydrogenase gene that is up-regulated by salt stress
Molecular cloning and characterization of rice 6-phosphogluconate dehydrogenase gene that is up-regulated by salt stress
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DOI：
10.1023/a:1026392422995
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发表时间：
2003-12-01
影响因子：
2.8
通讯作者：
Yang, JS
中科院分区：
文献类型：
--
作者：
Huang, J;Zhang, HS;Yang, JS
磷酸戊糖途径（PPP）是植物体内重要的代谢途径。本研究从水稻中分离到一个编码PPP关键酶6-磷酸葡萄糖酸脱氢酶（6-phosphogluconate dehydrogenase，6PGDH）的cDNA，命名为Os 6PGDH。Os 6PGDH编码蛋白是一种胞质同工酶，其N端缺少质体转运肽。该基因cDNA全长1751 bp，编码480个氨基酸，与玉米、菠菜和苜蓿6PGDH的同源性分别为94%、84%和83%。克隆的mRNA序列与水稻基因组计划的基因组序列的比较显示，在该基因的翻译区中没有内含子的简单的基因组组织。RT-PCR结果表明，Os 6PGDH在花序中表达量较高，在根和胚中表达量较低，而在叶片中几乎不表达。此外，Os 6PGDH在盐胁迫下在地上部中上调。因此，6-PGDH可能在植物细胞分裂和盐胁迫反应中起重要作用。
The pentose phosphate pathway (PPP) is the important metabolism pathway in plant. In the present study, a cDNA encoding one of the key enzymes of PPP, 6-phosphogluconate dehydrogenase( 6PGDH), was isolated from rice and designated as Os6PGDH. The Os6PGDH encoding protein is a cytosolic isoenzyme according to the absence of plastid transit peptide at the N-terminus. The full-length cDNA of 1751 bp encodes 480 amino acids and its putative protein sequence is 94%, 84% and 83% identical to maize, spinach and alfalfa 6PGDHs respectively. Comparison of the cloned mRNA sequence with that of the genomic sequence from the Rice Genome Project showed a simple genomic organization devoid of introns in the translated region of the gene. RT-PCR experiments revealed that Os6PGDH expression was high in inflorescence, low in root and embryos but almost absent in leaves. Furthermore, Os6PGDH was up-regulated in the shoots under salt stress. It is suggested that 6PGDH in plant may play an important role in cell division and salt response.