Synthesis of Barnase Site-Specifically Labelled with Two 13C Atoms Using Partially Protected Peptide Thioester Building Blocks
Synthesis of Barnase Site-Specifically Labelled with Two 13C Atoms Using Partially Protected Peptide Thioester Building Blocks
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使用部分保护的肽硫酯结构单元合成用两个 13C 原子位点特异性标记的 Barnase
DOI：
10.1246/bcsj.66.3004
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发表时间：
1993
影响因子：
4
通讯作者：
S. Aimoto
中科院分区：
文献类型：
--
作者：
H. Hojo;S. Aimoto
用两个13 C原子位点特异性标记的芽孢杆菌RNA酶，使用部分保护的肽硫酯作为结构单元合成。四个部分保护的肽段（Boc-[Lys（Boc）19，27]-芽孢杆菌RNA酶（1-34）-SC（CH 3）2CH 2CO-Nle-NH 2，iNoc-[Lys（Boc）39，49]-芽孢杆菌RNA酶（35-52）-SC（CH 3）2CH 2CO-Nle-NH 2，iNoc-[Lys（Boc）62，66]-芽孢杆菌RNA酶（53-81）-SC（CH 3）2CH 2CO-Nle-NH 2，[Lys（Boc）98，108]-芽孢杆菌RNA酶（82-110））在银离子和N-羟基琥珀酰亚胺（HONSu）存在下连续缩合。最后，以羧基端肽段为基准，以11%的产率获得了具有完全核糖核酸酶活性的芽孢杆菌RNA酶。
Barnase, site-specifically labelled with two 13C atoms, was synthesized using partially protected peptide thioesters as building blocks. Four partially protected peptide segments (Boc–[Lys(Boc)19,27]–barnase(1—34)–SC(CH3)2CH2CO–Nle–NH2, iNoc–[Lys(Boc)39,49]–barnase(35—52)–SC(CH3)2CH2CO–Nle–NH2, iNoc–[Lys(Boc)62,66]–barnase(53—81)–SC(CH3)2CH2CO–Nle–NH2, [Lys(Boc)98,108]–barnase(82—110)) were successively condensed in the presence of silver ions and N-hydroxysuccinimide (HONSu). Finally, barnase with full ribonuclease activity was obtained in a yield of 11% based on the carboxyl terminal peptide segment.