Platelet adhesion and release: interfacial concentration of released materials.
Platelet adhesion and release: interfacial concentration of released materials.
复制标题
血小板粘附和释放：释放物质的界面浓度。
DOI：
10.1152/ajpheart.1981.240.1.h99
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
I. Feuerstein
中科院分区：
文献类型：
--
作者：
G. Adams;I. Feuerstein
在管流中研究了血小板在胶原涂层玻璃上的沉积和从粘附血小板的释放。通过在含有白蛋白、腺苷三磷酸双磷酸酶和红细胞的台氏溶液中使用经51 Cr和[3H]血清素放射性标记的清洗猪血小板评价80和320 s-1之间的剪切速率。细胞积累是最高的管的入口处，并直接成比例的所有位置的暴露时间从入口到20厘米，时间长达800秒。入口处的释放最低，为60%，下游增加至90%。积累和释放信息作为计算的输入，该计算在管的表面产生ADP表面浓度值。这些范围从80 s-1时的0.6 microM到320 s-1时的1.8 microM。在浊度测定装置中，需要0.5、1.0和5.0 μ M的ADP浓度来诱导形状变化，分别为10%和80%的聚集。聚集也得到了亚阈值ADP和胶原的组合。我们推测，血小板的形状变化可能发生在表面附近，为粘附、聚集和在表面释放做准备，ADP和胶原协同作用。
Platelet deposition on collagen-coated glass and release from adherent platelets were studied in tube flow. Shear rates between 80 and 320 s-1 were evaluated by use of washed porcine platelets radiolabeled with 51Cr and [3H]serotonin in Tyrode solution containing albumin, apyrase, and red blood cells. Cell accumulation was highest at the tube's inlet and was directly proportional to exposure time for all positions up to 20 cm from the entrance and for times up to 800 s. Release was lowest at the inlet, 60%, and increased to 90% downstream. The accumulation and release information served as input to a calculation that yielded ADP surface concentration values at the tube's surface. These ranged from 0.6 microM at 80 s-1 to 1.8 microM at 320 s-1. In a turbidimetric device, ADP concentrations of 0.5, 1.0, and 5.0 microM were required to induce shape change, 10 and 80% aggregation, respectively. Aggregation was also obtained with subthreshold ADP and collagen in combination. We surmise that platelet shape change could have occurred adjacent to the surface in preparation for adhesion, aggregation, and release at the surface, and that ADP and collagen acted synergistically.