Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs.
Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs.
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DOI：
10.1186/1743-422x-8-201
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发表时间：
2011-05-02
期刊：
影响因子：
4.8
通讯作者：
Zhu YY
中科院分区：
文献类型：
--
作者：
Liu J;Fan XZ;Wang Q;Xu L;Zhao QZ;Huang W;Zhou YC;Tang B;Chen L;Zou XQ;Sha S;Zhu YY
猪瘟（Classical swine fever，CSF）是由猪瘟病毒（Classical swine fever virus，CSFV）引起的一种传染病，是国际兽疫局（OIE）规定的法定传染病。然而，目前对猪瘟病毒在猪体内的分布、组织嗜性、致病性、复制和排泄等方面的研究还很不够。本研究采用实时荧光定量RT-PCR和免疫组化技术研究了病毒在实验感染猪内脏器官中的动态分布和组织嗜性。建立了相对定量的实时荧光PCR方法，并用于检测实验感染猪内脏器官中的病毒载量。该研究显示，在感染后第1天至第8天从猪体内采集的所有21个内脏和血液中均检测到病毒，并且从感染后第1天至第8天病毒载量增加。然而，在感染后的前2天内，大多数内脏器官中的病毒载量分布不规则。血液、淋巴组织、胰腺和回肠通常含有最高的病毒载量，而心脏、十二指肠和脑显示相对较低的病毒载量。结果表明，实时荧光定量RT-PCR检测结果显示，猪瘟病毒在感染猪后第1 ~ 8天病毒载量呈上升趋势，与免疫组化染色结果一致。数据还显示，CSFV可能在血液、淋巴组织、胰腺和回肠中复制，而不太可能在心脏、十二指肠和脑中复制。本研究结果为进一步阐明CSFV在内脏器官的致病机制奠定了基础，提示血液、淋巴组织、胰腺和回肠可能是CSFV急性感染的首选部位。
Classical swine fever (CSF), caused by the Classical swine fever virus (CSFV), is an Office International des Epizooties (OIE) notifiable disease. However, we are far from fully understand the distribution, tissue tropism, pathogenesis, replication and excretion of CSFV in pigs. In this report, we investigated the dynamic distribution and tissue tropism of the virus in internal organs of the experimentally infected pigs using real-time RT-PCR and immunohistochemistry (IHC). A relative quantification real-time PCR was established and used to detect the virus load in internal organs of the experimentally infected pigs. The study revealed that the virus was detected in all 21 of the internal organs and blood collected from pigs at day 1 to day 8 post infections, and had an increasing virus load from day 1 to day 8 post infections. However, there was irregular distribution virus load in most internal organs over the first 2 days post infection. Blood, lymphoid tissue, pancreas and ileum usually contain the highest viral loads, while heart, duodenum and brain show relatively low viral loads. All the data suggest that CSFV had an increasing virus load from day 1 to day 8 post infections in experimentally infected pigs detected by real-time RT-PCR, which was in consistent with the result of the IHC staining. The data also show that CSFV was likely to reproduce in blood, lymphoid tissue, pancreas and the ileum, while unlikely to replicate in the heart, duodenum and brain. The results provide a foundation for further clarification of the pathogenic mechanism of CSFV in internal organs, and indicate that blood, lymphoid tissue, pancreas and ileum may be preferred sites of acute infection.