Release of DNA in circulating blood and induction of anti-DNA antibodies after injection of bacterial lipopolysaccharides.
Release of DNA in circulating blood and induction of anti-DNA antibodies after injection of bacterial lipopolysaccharides.
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DOI：
10.1084/jem.140.5.1189
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发表时间：
1974-11-01
期刊：
影响因子：
--
通讯作者：
Meischer PA
中科院分区：
文献类型：
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作者：
Fournié GJ;Lambert PH;Meischer PA
本实验结果表明，在注射细菌脂多糖(LPS)后，包括裸鼠在内的不同品系的C57BL/6裸鼠均能产生抗单链DNA和抗双链DNA抗体。这种抗DNA反应是剂量依赖的，并且随着注射的小鼠品系的不同而不同。它与H-2组织相容性基因座或对内毒素的免疫应答无关。脂A组分似乎是这种特殊作用的脂多糖分子的活性部分。此外，还发现在注射脂多糖几个小时后，循环血液中就会释放出DNA。释放的DNA大部分具有SS DNA的物理化学和免疫化学特征。因此，注射脂多糖诱导的抗DNA反应可能是在免疫系统，特别是B淋巴细胞能够对这种可溶性抗原产生免疫反应时，以一种特殊的免疫原性形式释放DNA的结果。内毒素的这些作用可能是在革兰氏阴性菌感染过程中触发或加剧前DNA抗体的原因，也可能与系统性红斑狼疮的发病机制有关。
The present data demonstrate the induction of antisingle-stranded (SS) DNA and antidouble-stranded DNA antibodies in various strains of mice, including athymic C57BL/6 nude mice, after the injection of bacterial lipopolysaccharide (LPS). This anti-DNA response is dose dependent and varies quantitatively according to the strain of the injected mice. It is not correlated to the H-2 histocompatibility locus nor to the immune response to LPS. The lipid A fraction appears to be the active part of the LPS molecule for this particular effect. In addition, it was found that DNA is released in circulating blood a few hours after the injection of LPS. Most of the DNA released has physicochemical and immunochemical characteristics of SS DNA. Therefore, the anti-DNA response induced by injections of LPS may be the result of a release of DNA in a particularly immunogenic form at a time when the immune system, in particular the B lymphocytes, is rendered capable by LPS of developing an immune response to such a soluble antigen. These effects of LPS may account for the triggering or the exacerbation of ante-DNA antibodies during infections with gram-negative bacteria, and a similar mechanism may be involved in the pathogenesis of systemic lupus erythematosus.