Increasing prevalence of Campylobacter jejuni in feedlot cattle through the feeding period
Increasing prevalence of Campylobacter jejuni in feedlot cattle through the feeding period
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DOI：
10.1128/aem.71.10.5752-5758.2005
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发表时间：
2005-10-01
影响因子：
4.4
通讯作者：
Hancock, DD
中科院分区：
文献类型：
--
作者：
Besser, TE;LeJeune, JT;Hancock, DD
通过反复的粪便细菌培养，监测了商品饲养场牛的空肠弯曲杆菌的流行情况。从进入饲养场开始，一直持续到屠宰，每隔2周采样20个饲养场围栏的粪便（n = 10）。最小二乘平均空肠梭菌患病率从第一次采样时的1.6%上升到屠宰前最后一次采样时的61.3%。在牛分离株中鉴定出不同的空肠大肠杆菌脉冲场凝胶电泳大限制谱（MRP），但5种常见的MRP和小变异占所有分型菌株的约80%。一半猪圈水槽用水的氯化处理对牛空肠弧菌的流行没有影响。总体而言，氯化围栏和非氯化围栏的最小二乘平均空肠弧菌患病率分别为45.6%和43.6%。本研究结果表明，在饲养期间，空肠梭菌在饲养场牛之间明显传播，不受水氯化的影响，导致牛在接近屠宰时排泄空肠梭菌的高流行率。
The prevalence of Campylobacter jejuni in commercial feedlot cattle was monitored throughout the feeding period by repeated bacteriologic culture of feces. Fecal pats (n = 10) in 20 feedlot pens were sampled at 2-weeks interval beginning at entry into the feedlot and continuing until slaughter. The least-squares mean C. jejuni prevalence increased from 1.6% at the first sampling to 61.3% at the final sampling just prior to slaughter. Diverse C. jejuni pulsed-field gel electrophoresis macrorestriction profiles (MRP) were identified among the cattle isolates, but five prevalent MRP and minor variants accounted for > 80% of all typed isolates. Chlorination of the water supplied to the water troughs of half of the pens did not affect C.jejuni prevalence in the cattle. Overall, the least-squares mean C.jejuni prevalences were 45.6 and 43.6% in chlorinated and nonchlorinated feedlot pens, respectively. The results of this study demonstrate apparent transmission of C. jejuni among feedlot cattle during the feeding period, unaffected by water chlorination, resulting in a high prevalence of C. jejuni excretion by cattle approaching slaughter.