Two possible conducting states of the influenza A virus M2 ion channel.
Two possible conducting states of the influenza A virus M2 ion channel.
复制标题
甲型流感病毒 M2 离子通道的两种可能的传导状态。
DOI：
10.1016/s0014-5793(00)01522-2
复制
发表时间：
2000
期刊：
影响因子：
3.5
通讯作者：
Klein,ML
中科院分区：
文献类型：
--
作者：
Zhong,Q;Newns,DM;Pattnaik,P;Lear,JD;Klein,ML
基于甲型流感病毒M2通道的25个残基的Duff-Ashley跨膜序列，对质子化的四螺旋束进行了分子动力学模拟。研究了平衡良好的四聚通道，其中一个、两个和四个H37残基被质子化。质子化的多肽束被浸泡在水/辛烷相分离体系的辛烷部分中，这提供了一个类似膜的环境。模拟表明，对应于含有一个或两个质子化组氨酸的四聚体，M2通道可能存在两个导通状态。双质子态的更开放的结构表明它具有更高的电导。
Molecular dynamics simulations have been performed on protonated four-helix bundles based on the 25-residue Duff–Ashley transmembrane sequence of the M2 channel of the influenza A virus. Well-equilibrated tetrameric channels, with one, two and four of the H37 residues protonated, were investigated. The protonated peptide bundles were immersed in the octane portion of a phase-separated water/octane system, which provided a membrane-mimetic environment. The simulations suggest that there could be two conducting states of the M2 channel corresponding to tetramers containing one or two protonated histidines. The more open structure of the doubly protonated state suggests it would have the higher conductance.