Gelatin Nanoparticles
Gelatin Nanoparticles
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DOI：
10.1007/978-1-4939-9516-5_6
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Metwally, Abdelkader A.
中科院分区：
文献类型：
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作者：
Hathout, Rania M.;Metwally, Abdelkader A.
目前，明胶纳米颗粒在所有可用的几种给药途径中的药物和基因递送中获得更多的理由。然而，由于明胶质量根据其来源的变化以及由于其可变的分子量，这种类型的纳米颗粒的均匀且分散性较低的制备仍然是一项具有挑战性的任务。因此，提出了几种方法，其中双重去溶剂化方法已被证明可以产生关于粒度和均匀性的最佳结果。因此，我们在本章中描述了这种方法的详细过程。我们还介绍了我们的方案，阳离子化的这种纳米粒子，因为它是广泛需要的情况下，负载遗传物质或蛋白质。此外，还逐步介绍了通常用于成像或生物分布研究目的的明胶纳米颗粒的FITC标记。
Currently, gelatin nanoparticles are gaining more grounds in drug and gene delivery throughout all the available several routes of administration. Yet, the homogenous and less disperse preparation of this type of nanoparticles is still a challenging task due to the variation of the gelatin quality according to its source and due to its variable molecular weight. Accordingly, several methods were proposed from which the double-desolvation method has been proven to yield optimum results regarding particle size and homogeneity. Thereby, we describe in this chapter a detailed procedure of this method. We also introduce our protocols of the cationization of this kind of nanoparticles as it is extensively needed in case of loading genetic materials or proteins. Additionally, FITC labeling of gelatin nanoparticles that is usually utilized for purposes of imaging or bio-distribution studies is also introduced step by step.