Epitope mapping of HTLV envelope seroreactivity in LGL leukaemia
Epitope mapping of HTLV envelope seroreactivity in LGL leukaemia
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DOI：
10.1046/j.1365-2141.1998.00691.x
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发表时间：
1998-05
影响因子：
6.5
通讯作者：
T. Loughran, JR;K. Hadlock;R. Perzova;T. Gentile;Qing Yang;S. Foung;B. Poiesz
中科院分区：
文献类型：
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作者：
T. Loughran, JR;K. Hadlock;R. Perzova;T. Gentile;Qing Yang;S. Foung;B. Poiesz
大约50%的大颗粒淋巴细胞(LGL)白血病患者的血清与重组人T细胞白血病/淋巴瘤病毒(HTLV)跨膜蛋白p21e反应。通过与重组蛋白GD21和BA21的反应，确定了env p21e中的两个免疫优势表位。在这项研究中，检测了41例LGL白血病患者血清对这些重组HTLV env蛋白的反应性。总体而言，21/41(51%)血清对p21e有反应。只有两份血清对GD21有反应。P21e的主要免疫反应是针对BA21表位，其中19/41(46%)的血清BA21阳性。血清转换为BA21蛋白也被记录在案。聚合酶链式反应分析证实典型HTLV序列发生率低(2/41，5%)。这些数据证明了BA21血清反应性与LGL白血病之间的联系。这一发现提出了这样一种可能性，即这种BA21血清反应性可能是由于与HTLV跨膜糖蛋白有一定氨基酸同源性的细胞或逆转录病毒抗原发生交叉反应所致。
Sera from approximately 50% of patients with large granular lymphocyte (LGL) leukaemia react with a recombinant human T‐cell leukaemia/lymphoma virus (HTLV) transmembrane envelope protein, p21e. Two immunodominant epitopes within env p21e have been defined by reactivity against recombinant proteins GD21 and BA21. In this study sera from 41 patients with LGL leukaemia were examined for reactivity against these recombinant HTLV env proteins. Overall, 21/41 (51%) sera reacted to p21e. Only two sera reacted to GD21. The predominant immunoreactivity against p21e was directed against the BA21 epitope, with 19/41 (46%) sera being BA21 positive. Seroconversion to BA21 protein was also documented. PCR analyses confirmed the low incidence of protypical HTLV sequences (2/41, 5%). These data document an association between BA21 seroreactivity and LGL leukaemia. This finding raises the possibility that such BA21 seroreactivity could be due to cross‐reactivity to a cellular or retroviral antigen sharing some amino acid homology with the transmembrane glycoprotein of HTLV.