Evaluation of mobile phase, ion pairing, and temperature influence on an HILIC-MS/MS method for L-arginine and its dimethylated derivatives detection.
Evaluation of mobile phase, ion pairing, and temperature influence on an HILIC-MS/MS method for L-arginine and its dimethylated derivatives detection.
复制标题
DOI：
10.1002/jssc.200800142
复制
发表时间：
2008-07
影响因子：
3.1
通讯作者：
G. Paglia;O. D’Apolito;F. Tricarico;Daniela Garofalo;G. Corso
中科院分区：
文献类型：
--
作者：
G. Paglia;O. D’Apolito;F. Tricarico;Daniela Garofalo;G. Corso
不对称N（G），-N（G）-二甲基精氨酸（ADMA）在肾衰竭、糖尿病和高胆固醇血症等疾病中增加。探讨了亲水作用色谱法（HILIC）分离游离L-精氨酸（Arg）、ADMA和对称N（G），-N（G '）-二甲基精氨酸（SDMA）的可行性和实用性，并考察了一些实验参数对这些分析物色谱行为的影响。在开发一种快速简便的HILIC-MS/MS方法的过程中，研究了移动的相中水和TFA含量以及柱温的影响，该方法可能适用于定量血浆中游离Arg、ADMA和SDMA的常规分析。我们的结果表明，使用ACN/水（90：10）移动的流动相，TFA%作为添加剂的范围为0.015 - 0.025%，柱温范围为25 - 30 ℃，可在效率和峰形之间实现良好的折衷，保留时间和总色谱运行时间可接受。
Asymmetric N(G),-N(G)-dimethylarginine (ADMA) increases in diseases such as renal failure, diabetes mellitus, and hypercholesterolemia. The feasibility and utility of a hydrophilic interaction chromatography (HILIC) method for the separation of free L-arginine (Arg), ADMA, and symmetric N(G),-N(G')-dimethylarginine (SDMA) on a typical silica column were explored and the impact of some experimental parameters on the chromatographic behavior of these analytes was investigated. The effect of water and TFA content in mobile phase and of column temperature was investigated during the development of a fast and simple HILIC-MS/MS method that might be suitable for the quantification of free Arg, ADMA, and SDMA in plasma for routine analysis. Our results show that a good compromise between efficiency and peak shape with acceptable retention and total chromatographic run time is achieved using an ACN/water (90:10) mobile phase with TFA% as additive ranging from 0.015 to 0.025% and column temperature ranging from 25 to 30 degrees C.