Opposite patterns in the annual distribution and time-course of endogenous abscisic acid and indole-3-acetic acid in relation to the periodicity of cambial activity in Eucommia ulmoides Oliv.
Opposite patterns in the annual distribution and time-course of endogenous abscisic acid and indole-3-acetic acid in relation to the periodicity of cambial activity in Eucommia ulmoides Oliv.
复制标题
DOI：
10.1093/jxb/eri095
复制
发表时间：
2005-03
影响因子：
6.9
通讯作者：
Kalima-N'koma Mwange;H. Hou;You-Qun Wang;Xin He;K. Cui
中科院分区：
文献类型：
--
作者：
Kalima-N'koma Mwange;H. Hou;You-Qun Wang;Xin He;K. Cui
通过使用多克隆和罗丹明红荧光二抗的 ABA 和 IAA 免疫定位、使用高效液相色谱 (HPLC) 的 ABA 和 IAA 定量以及血管细胞的系统监测，研究了杜仲树中游离脱落酸 (ABA) 和吲哚 3-乙酸 (IAA) 的季节变化及其与形成层活性的关系。 层生产。 ABA 和 IAA 明显表现出相反的年度分布模式。在活跃期（AP），免疫定位和 HPLC 均检测到 ABA 突然下降，达到夏季的最低水平。休眠期间，ABA在第一个静止期（Q1）（秋季）开始增加，在其余时间（冬季）达到峰值，并从第二个静止期（Q2）（冬末）开始逐渐下降。 IAA 表现出与 ABA 相反的模式：AP 急剧增加，但从第一季度开始明显下降。纵向上，ABA 分布从顶部到基底增加，与 IAA 形成对比。从侧面看，大部分 ABA 位于成熟的维管组织中，而 IAA 则主要发生在形成层区域。维管组织中伴随的 IAA-ABA 分布和季节变化与木质部和韧皮部细胞的产生以及后期木材分化和成熟密切相关。有趣的是，在水培系统中，将外源 ABA 应用于静止的 E. ulmoides 枝条，抑制了外部 IAA 对形成层活性重新激活的作用。这些结果表明，在 E. ulmoides 中，ABA 和 IAA 可能在形成层区域相互作用。年度形成层活动可能受到 IAA:ABA 比率的影响； ABA可能在高等植物维管形成层休眠中发挥关键作用。还讨论了荷尔蒙变化与 E. ulmoides 的（特定）年度生命周期之间的关系。
The seasonal change of free abscisic acid (ABA) and indole-3-acetic acid (IAA) and their relationship with the cambial activity in Eucommia ulmoides trees were investigated by ABA and IAA immunolocalization using primary polyclonal and rhodamine-red fluorescing secondary antibodies, ABA and IAA quantification using high performance liquid chromatography (HPLC), and systematic monitoring of vascular cell layers production. ABA and IAA clearly displayed opposite annual distribution patterns. In the active period (AP), both immunolocalization and HPLC detected an abrupt decrease of ABA, reaching its lowest level in the summer. During dormancy, ABA started increasing in the first quiescence (Q1) (autumn), peaked in the rest (winter), and gradually decreased from the onset of the second quiescence (Q2) (the end of winter). IAA showed a reverse pattern to that of ABA: it sharply increased in AP, but noticeably decreased from the commencement of Q1. Longitudinally, the ABA distribution increased apico-basally, contrasting with IAA. Laterally, most of the ABA was located in mature vascular tissues, whereas the IAA essentially occurred in the cambial region. The concomitant IAA-ABA distribution and seasonal changes in vascular tissues greatly correlated with xylem and phloem cell production, and late wood differentiation and maturation. Interestingly, the application of exogenous ABA to quiescent E. ulmoides branches, in a water-culture system, inhibited external IAA action on cambial activity reactivation. These results suggest that, in E. ulmoides, ABA and IAA might probably interact in the cambial region. The annual cambial activity could be influenced by an IAA:ABA ratio; and ABA might play a key role in vascular cambium dormancy in higher plants. The relationship between hormonal changes and the (particular) annual life cycle of E. ulmoides is also discussed.