Confocal endomicroscopic imaging of normal and neoplastic human tongue tissue using ALA-induced-PPIX fluorescence: a preliminary study.
Confocal endomicroscopic imaging of normal and neoplastic human tongue tissue using ALA-induced-PPIX fluorescence: a preliminary study.
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使用 ALA 诱导的 PPIX 荧光对正常和肿瘤性人舌组织进行共焦内显微成像：一项初步研究。
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发表时间：
2004
期刊：
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通讯作者：
K. Soo
中科院分区：
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作者：
Wei Zheng;M. Harris;K. Kho;P. Thong;A. Hibbs;M. Olivo;K. Soo
共聚焦显微内镜是一种新型的非侵入性显微技术，能够对活体组织或细胞进行表面和亚表面成像。本研究的目的是评估利用刚性共聚焦显微内镜（RCE）系统的可能性，该系统开发用于检测与5-氨基乙酰丙酸（ALA）诱导的内源性原卟啉IX（PPIX）荧光相结合的正常和肿瘤人舌组织的形态学变化。用氩离子激光激发波长为488 nm，组织荧光检测波长在515 nm以上的新型RCE系统对3例舌鳞状细胞癌（SCC）患者进行了检查。将0.4%的5-ALA冲洗溶液局部应用于患者的口腔粘膜约15分钟，然后在90-120分钟的最佳孵育期后，在舌的病变部位上进行组织PPIX荧光的共聚焦显微内镜成像。ALA-PPIX荧光共聚焦显微内镜成像也进行了正常网站的舌头在体内从两个健康志愿者。使用连接到RCE探头的特殊设计的组织稳定器，可以最大限度地减少组织运动引起的图像失真。可以实时获取舌的良好质量的ALA介导的共焦荧光图像，提供明确的微形态结构（例如，丝状乳头、角化上皮和菌状乳头）。使用RCE成像还可以清楚地识别与癌症转化相关的口腔组织中的组织结构的变化。在这项研究中获得的初步结果表明，ALA介导的刚性共聚焦显微内镜可能有一个显着的潜力，快速，非侵入性的诊断和评价早期口腔癌在体内。
Confocal endomicroscopy is a novel and non-invasive microscopic technique that enables surface and subsurface imaging of living tissues or cells in vivo. The purpose of this study was to assess the possibility of utilizing a rigid confocal endomicroscope (RCE) system developed for detecting morphological changes in living normal and neoplastic human tongue tissue in combination with 5-aminolevulinic acid (ALA)-induced endogenous protoporphyrin IX (PPIX) fluorescence. Three patients with squamous cell carcinoma (SCC) of the tongue were examined using the novel RCE system with the excitation wavelength at 488 nm from an argon-ion laser and the detection wavelengths of the tissue fluorescence above 515 nm. Patients were topically applied with 0.4% of 5-ALA rinsing solution to the oral mucosa for approximately 15 min, and then the confocal endomicroscopic imaging of tissue PPIX fluorescence was performed on the lesion sites of the tongue after an optimal incubation period of 90-120 min. For comparison purposes, ALA-PPIX fluorescence confocal endomicroscopic imaging was also carried out on the normal sites of the tongue in vivo from two healthy volunteers. Image distortions due to tissue motion can be minimized using a specially designed tissue stabilizer attached to the RCE probe. Good quality ALA-mediated confocal fluorescence images of the tongue can be acquired in real-time, providing well-defined micro-morphological structures (e.g., filiform papillae, keratinized epithelium and fungiform papillae) of the tongue in vivo. Changes of tissue structures in oral tissue associated with cancer transformation can also be clearly identified using the RCE imaging. Preliminary results obtained in this study suggest that ALA-mediated rigid confocal endomicroscopy may have a significant potential for the rapid, non-invasive diagnosis and evaluation of early oral cancers in vivo.