Necroptosis contributes to the NMDA-induced excitotoxicity in rat's cultured cortical neurons
Necroptosis contributes to the NMDA-induced excitotoxicity in rat's cultured cortical neurons
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DOI：
10.1016/j.neulet.2008.08.037
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发表时间：
2008-12-12
影响因子：
2.5
通讯作者：
Zhang, Ce
中科院分区：
文献类型：
--
作者：
Li, Yanli;Yang, Xiaorong;Zhang, Ce
坏死性凋亡是一种新发现的细胞死亡类型，其特征在于坏死和凋亡的生物化学和超微结构特征。据报道，NEC-1表现出对坏死性凋亡的选择性抑制，其现在已被用作坏死性凋亡的操作定义。本研究的目的是评估坏死性凋亡是否参与NMDA诱导的兴奋性毒性，以及细胞内Ca 2+的升高是否参与NMDA诱导的坏死性凋亡。结果表明，Nec-1（100 μ mol/L）可抑制NMDA诱导的细胞存活率下降26%（p < 0.01），抑制NMDA诱导的活细胞减少23%（p < 0.01），并使NMDA诱导的LDH漏出减少28%（p < 0.01）。此外，Nec-1还可抑制NMDA诱导的细胞内Ca ~（2+）升高36%（p
Necroptosis is a newly discovered type of cell death characterized with the combined biochemical and ultrastructural features of necrosis and apoptosis. Nec-1 has been reported to exhibit the selective inhibition for necroptosis, which has now been used as an operational definition of necroptosis. The purpose of this study was to evaluate whether necroptosis is involved in the NMDA-induced excitotoxicity and furthermore whether the elevation of intracellular Ca2+ is involved in the NMDA-induced necroptosis. Our findings showed that Nec-1 (100 mu mol/L) inhibited NMDA-induced decrease of cell viability by 26% (p < 0.01), suppressed NMDA-induced decrease of living cells by 23% (p < 0.01) and attenuated NMDA-induced leakage of LDH by 28% (p < 0.01). In addition, Nec-1 also suppressed NMDA-induced elevation of intracellular Ca2+ by 36% (p