In vitro studies of human and rat osteoclast activity on hydroxyapatite, β-tricalcium phosphate, calcium carbonate
In vitro studies of human and rat osteoclast activity on hydroxyapatite, β-tricalcium phosphate, calcium carbonate
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DOI：
10.1016/s1389-0344(02)00023-0
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发表时间：
2002-08-01
期刊：
影响因子：
--
通讯作者：
Hildebrand, HF
中科院分区：
文献类型：
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作者：
Monchau, F;Lefèvre, A;Hildebrand, HF
对破骨细胞引起的陶瓷降解的研究旨在评估破骨细胞与陶瓷的相互作用，并确定哪种陶瓷更适合用作骨替代品。本研究考察了破骨细胞对不同溶解度陶瓷的吸收活性。采用羟基磷灰石（HA）、β -磷酸三钙（TCP）和碳酸钙（方解石）三种不同的生物陶瓷对新生大鼠和人巨细胞瘤的破骨细胞进行培养。肌动蛋白标记显示细胞骨架，扫描电镜观察陶瓷表面。在所有材料上，肌动蛋白呈典型环状分布。扫描电镜检查表明，不同陶瓷的吸收腔在形状和深度上有明显的差异。在纯HA上，浅表攻击，清晰可见，但很少扩展。在纯β - tcp上观察到大量深且划分良好的吸收空洞，但也不太及时地检测到攻击。在纯方解石上，发现了一种具有尖刺形式的攻击，非常广泛但很肤浅。降解测量显示，在破骨细胞存在的情况下，磷钙陶瓷和所有陶瓷的钙释放量显著增加。两种细胞模型均具有上述特点，大鼠破骨细胞也是研究陶瓷吸收的良好模型。(C) 2002 Elsevier Science B.V.版权所有
Investigations on the ceramic degradation caused by osteoclasts are designed to assess osteoclast-ceramic interactions and to determine which ceramics are more suitable for use as bone substitute. This study investigated the resorptive activity of osteoclasts on ceramics presenting different solubility rates. Osteoclasts isolated from new-born rat and from human giant cell tumour were cultured on different bioceramics: hydroxyapatite (HA), beta-tricalcium phosphate (TCP) and calcium carbonate (calcite). Cytoskeletal was revealed by actin labelling and ceramic surfaces were observed by scanning electron microscopy (SEM). On all materials, the distribution of actin in typical ring was revealed. SEM examinations showed a clear difference in the shape and the depth of resorption lacunae on different ceramics. On pure HA, a superficial attack, clearly visible but very little extended. Numerous resorption lacunae, deep and well-delimited were observed on pure beta-TCP, but attacks less punctually were detected too. On pure calcite, an attack with form of spikes, very widespread but superficial was revealed. Degradation measurements revealed a significant increase of P release from the phosphocalcic ceramics and of Ca from all ceramics in the presence of osteoclasts. The both cell models found these characteristics, the rat osteoclasts were also an excellent model to study the ceramic resorption. (C) 2002 Elsevier Science B.V. All rights reserved.