The role of cyanide-resistant respiration in Solanum tuberosum L. against high light stress
The role of cyanide-resistant respiration in Solanum tuberosum L. against high light stress
复制标题
氰化物在马铃薯中抵抗呼吸的作用。
DOI：
10.1111/plb.13098
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发表时间：
2020-05-01
期刊：
影响因子：
3.9
通讯作者：
Li, H.
中科院分区：
文献类型：
--
作者：
Hua, D.;Ma, M.;Li, H.
抗氰呼吸是马铃薯线粒体能量耗散的重要途径。它可以被强光激活;然而，目前还不清楚抗氰呼吸在马铃薯对强光胁迫的响应中起什么作用。我们设计了马铃薯抗氰呼吸途径功能基因StAOX的CRISPR载体。农杆菌GV 3101转化马铃薯。测定了强光胁迫下马铃薯叶片过氧化氢水平、丙二醛含量、抗氧化活性和抗氰呼吸能力。光合效率和叶绿素含量的测定。此外，还考察了苹果酸-草酰乙酸穿梭路线的运行情况以及光呼吸相关酶的转录水平，结果表明，转化马铃薯叶片上的测序目标位点发生了两个碱基替换。结果表明，转基因马铃薯叶片中活性氧的积累和膜脂过氧化作用增强，光合效率降低。苹果酸-草酰乙酸穿梭途径和光呼吸相关酶的转录水平也显著提高，表明抗氰呼吸是马铃薯响应强光胁迫的重要生理途径。植物抗氰呼吸与光合作用密切相关。这暗示了植物抗氰呼吸在植物光合作用、能量转换和碳骨架形成中的重要性。
Cyanide-resistant respiration in potato mitochondria is an important pathway for energy dissipation. It can be activated by high light; however, it is unclear what roles cyanide-resistant respiration plays in the response to high light stress in potato.We designed a CRISPR vector for the functional gene StAOX of the potato cyanide-resistant respiratory pathway. Agrobacterium tumefaciens GV3101 was transformed into potato. Hydrogen peroxide level, MDA content, antioxidant activity and cyanide-resistant respiratory capacity of potato leaves under high light stress were determined. Photosynthetic efficiency and chlorophyll content were determined. In addition, the operation of the malate-oxaloacetate shuttle route and transcription level of photorespiration-related enzymes were also examined.The results showed that two base substitutions occurred at the sequencing target site on leaves of the transformed potato. Accumulation of ROS and increased membrane lipid peroxidation were detected in the transformed potato leaves and lower photosynthetic efficiency was observed. The transcription level of the malate-oxaloacetate shuttle route and photorespiration-related enzymes also significantly increased.These results indicate that the cyanide-resistant respiration is an important physiological pathway in potato in response to high light stress. It also suggests that plant cyanide-resistant respiration is closely related to photosynthesis. This implies the unexplored importance of plant cyanide-resistant respiration in plant photosynthesis, energy conversion and carbon skeleton formation.