Treated dentin matrix particles combined with dental follicle cell sheet stimulate periodontal regeneration
Treated dentin matrix particles combined with dental follicle cell sheet stimulate periodontal regeneration
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处理过的牙本质基质颗粒与牙囊细胞片结合刺激牙周再生
DOI：
10.1016/j.dental.2019.05.016
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发表时间：
2019
期刊：
影响因子：
5
通讯作者：
Li Song
中科院分区：
文献类型：
--
作者：
Yang Hefeng;Li Jie;Hu Yu;Sun Jingjing;Guo Weihua;Li Hui;Chen Jinglong;Huo Fangjun;Tian Weidong;Li Song
目的牙周组织工程是恢复牙周支持结构和功能的一种有吸引力的方法。然而，完整的牙周再生尚未实现。先前的研究证明了使用细胞片和处理过的牙本质基质（TDM）再生生物牙根的可行性。方法在本研究中，我们使用细胞片与TDM颗粒（TDMP）相结合来再生牙周组织。分离并表征了人牙囊细胞 (hDFC)。制备并表征了人牙囊细胞片 (hDFCS) 和人 TDMP (hTDMP)。体外评估了 hTDMP 对人骨髓基质细胞 (hBMSC) 的成骨作用，并在体内评估了大鼠颅骨骨缺损的成骨作用。通过实时PCR、蛋白质印迹、放射线分析和组织学分析来评估hTDMP的牙周诱导能力。制备单壁牙周骨内缺损，评价TDMP/DFCSs对Beagle犬的牙周再生能力。结果结果表明hDFCs为间充质干细胞。 hTDMP促进hBMSCs的增殖和成骨分化。 hTDMP 组和羟基磷灰石/β-磷酸三钙组的大鼠颅骨骨缺损区均观察到新骨形成。与其他组相比，犬 TDMP + DFCS 组的牙周样组织表现出更好的再生能力。意义这些结果证明了使用 TDMP/DFCS 在牙周再生中的潜力。
ObjectivePeriodontal tissue engineering is an attractive approach for restoring periodontal-supporting structures and functions. However, complete periodontal regeneration has not been accomplished. Previous studies demonstrated the feasibility of using cell sheets and treated dentin matrix (TDM) to regenerate bio-roots.MethodsIn this study, we regenerated periodontal tissue using cell sheets combined with TDM particles (TDMPs). Human dental follicle cells (hDFCs) were isolated and characterized. Human dental follicle cells sheets (hDFCSs) and human TDMPs (hTDMP) were fabricated and characterized. The osteogenic effect of hTDMP was evaluated on human bone marrow stromal cells (hBMSCs)in vitroand a rat calvarial bone defectin vivo. Real-time PCR, western blotting, radiograph analysis, and histological analysis were performed to evaluate the periodontal induction capacity of hTDMP. One-wall periodontal intrabony defects were prepared to evaluate the periodontal regeneration capacity of TDMP/DFCSs on beagle dogs.ResultsThe results showed that hDFCs were mesenchymal stem cells. hTDMP promoted the proliferation and osteogenic differentiation of hBMSCs. New bone formation was observed in the rat calvarial bone defect zone in both the hTDMP and hydroxyapatite/β-tricalcium phosphate groups. Periodontal-like tissues showed better regeneration in the canine TDMP + DFCS group than in the other groups.SignificanceThese results demonstrate the potential of using TDMP/DFCSs in periodontal regeneration.