P-glycoprotein and its inducible expression in three bivalve species after exposure to Prorocentrum lima
P-glycoprotein and its inducible expression in three bivalve species after exposure to Prorocentrum lima
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DOI：
10.1016/j.aquatox.2015.10.012
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发表时间：
2015-12-01
影响因子：
4.5
通讯作者：
Yang, Wei-Dong
中科院分区：
文献类型：
--
作者：
Huang, Lu;Liu, Su-Li;Yang, Wei-Dong
P-糖蛋白(P-gp或ABCB1)属于三磷酸腺苷结合盒(ABC)转运体家族，与水生生物的多重异源耐药(MXR)有关。为了提供贝类中P-gp的更多信息，本研究克隆了菲律宾蛤仔(Ruditapes Philippinarum)、毛虾(Scapharca Subcrenata)和泥螺(Tegillarca Ranosa)3种双壳类中P-gp的全长基因，并检测了其在贝类的鳃、消化腺、内收肌和外套膜中的表达。菲律宾链霉菌、枯枝杆菌和颗粒菌P-gp的全序列与其他物种的MDR/P-gp/ABCB蛋白有很高的同源性，具有典型的ABCB家族转运蛋白的序列结构。系统发育树分析表明，松材线虫和颗粒菌的P-gp氨基酸序列的亲缘关系最近，形成了一个独立的分支，然后与加州贻贝、高氏贻贝和长牡蛎聚为一支。然而，菲律宾对虾的P-gp基因序列与近缘海兔的同源物的相似性要高于与亚洲对虾和颗粒对虾的同源物，这表明双壳类的P-gp可能具有不同的同源序列。P-糖蛋白在所有被检测组织中均有表达，但两者之间存在较大差异。利马疫霉暴露后，P-gp在同一物种内和不同物种间的四种组织中的表达都有不同程度的变化，但其mRNA和蛋白质水平的变化并不总是同步的。(C)2015爱思唯尔B.V.保留所有权利。
P-glycoprotein (P-gp or ABCB1) belongs to the family of ATP-binding cassette (ABC) transporters responsible for multixenobiotic resistance (MXR) in aquatic organisms. To provide more information of P-gp in shellfish, in this study, complete cDNA of P-gp in three bivalve species including Ruditapes philippinarum, Scapharca subcrenata and Tegillarca granosa were cloned and its expressions in gill, digestive gland, adductor muscle and mantle of the three bivalves were detected after exposure to Prorocentrum lima, a toxogenic dinoflagellate. The complete sequences of R. philippinarum, S. subcrenata and T. granosa P-gp showed high homology with MDR/P-gp/ABCB proteins from other species, having a typical sequence organization as full transporters from the ABCB family. Phylogenetic analyses revealed that the amino acid sequences of P-gp from S. subcrenata and T. granosa had a closest relationship, forming an independent branch, then grouping into the other branch with Mytilus californianus, Mytilus galloprovincialis and Crassostrea gigas. However, P-gp sequences from R. philippinarum were more similar to the homologs from the more distantly related Aplysia californica than to homologs from S. subcrenata and T. granosa, suggesting that bivalves P-gp might have different paralogs. P-glycoprotein expressed in all detected tissues but there were large differences between them. After exposure to P. lima, the expression of P-gp changed in the four tissues in varying degrees within the same species and between different species, but the changes in mRNA and protein level were not always synchronous. (C) 2015 Elsevier B.V. All rights reserved.