Initiation, labile, and stabilization phases of experience-dependent plasticity at neocortical synapses.
Initiation, labile, and stabilization phases of experience-dependent plasticity at neocortical synapses.
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DOI：
10.1523/jneurosci.3575-12.2013
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发表时间：
2013-05-08
期刊：
影响因子：
--
通讯作者：
Barth AL
中科院分区：
文献类型：
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作者：
Wen JA;DeBlois MC;Barth AL
感觉输入的改变可以改变新皮层突触的强度。选择性激活的一个子集的胡须是足以加强层4-层2/3兴奋性突触在小鼠体感（桶）皮层，一个过程是NMDAR依赖性。通过分析感觉诱导的突触变化的时间过程，我们已经确定了体内突触强化的三个不同阶段。经过早期，NMDAR依赖性阶段，选择性晶须激活迅速转化为增加突触强度，我们确定了第二个阶段，这种增强作用是深刻的输入特定的，NMDAR依赖性抑郁症减少。这个不稳定阶段是短暂的，只持续几个小时，可能需要持续的感觉输入来减弱突触。残余突触强度维持在第三阶段，稳定阶段，这需要mGluR 5信号传导。这三个阶段的鉴定将促进调节突触不稳定性和稳定性的通路的分子解剖，并提出调节学习的潜在方法。
Alteration of sensory input can change the strength of neocortical synapses. Selective activation of a subset of whiskers is sufficient to potentiate layer 4-layer 2/3 excitatory synapses in the mouse somatosensory (barrel) cortex, a process that is NMDAR-dependent. By analyzing the time-course of sensory-induced synaptic change, we have identified three distinct phases for synaptic strengthening in vivo. After an early, NMDAR-dependent phase where selective whisker activation is rapidly translated into increased synaptic strength, we identify a second phase where this potentiation is profoundly reduced by an input-specific, NMDAR-dependent depression. This labile phase is transient, lasting only a few hours, and may require ongoing sensory input for synaptic weakening. Residual synaptic strength is maintained in a third phase, the stabilization phase, which requires mGluR5 signaling. Identification of these three phases will facilitate a molecular dissection of the pathways that regulate synaptic lability and stabilization, and suggest potential approaches to modulate learning.