Specific Detection and Simultaneously Localized Photothermal Treatment of Cancer Cells Using Layer-by-Layer Assembled Multifunctional Nanoparticles
Specific Detection and Simultaneously Localized Photothermal Treatment of Cancer Cells Using Layer-by-Layer Assembled Multifunctional Nanoparticles
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使用层层组装的多功能纳米粒子对癌细胞进行特异性检测和同步局部光热治疗
DOI：
10.1021/am405924g
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发表时间：
2014-05-14
影响因子：
9.5
通讯作者：
Liu, Jian
中科院分区：
文献类型：
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作者：
Shen, Jianwei;Li, Kunyang;Liu, Jian
非常需要开发多功能纳米粒子（MFNP），用于同时进行基于癌症生物标志物的检测和高选择性治疗。在这里，我们描述了一种与抗 HER2 单克隆抗体缀合的逐层组装 MFNP 的简便方法，证明了对乳腺癌 BT474 细胞（生物标志物 HER2 阳性）的特异性检测具有高信噪比。 MFNPs 包含清晰的 UCNP@Fe3O4@Au 核壳结构，由聚乙二醇 (PEG) 和抗 HER2 抗体包被，在各种水溶液中表现出优异的分散性。这种纳米颗粒和配体分子的独特组合使我们能够对癌细胞进行光热治疗 (PTT)，同时量化抗原抗体结合事件诱导的 MFNP 在癌细胞表面的分布。一个重要的发现是，彼此相邻或物理距离在微米以内的癌细胞可能在 PTT 中具有不同的生存或死亡命运。由于肿瘤细胞异质性，这种显着差异是由 MFNP 和细胞界面上的抗原抗体结合事件决定的。因此，我们的实验揭示了近红外激光连续波照射的单细胞水平上光热效应高度局部特征的新尺度。
There is a great need to develop multifunctional nanoparticles (MFNPs) for cancer biomarker-based detection and highly selective therapeutic treatment simultaneously. Here we describe a facile approach of layer-by-layer-assembled MFNPs conjugated with monoclonal antibody anti-HER2, demonstrating the specific detection of breast cancer BT474 cells (biomarker HER2 positive) with a high signal-to-noise ratio. The MFNPs contain a well-defined core shell structure of UCNP@Fe3O4@Au coated by poly(ethylene glycol) (PEG) and anti-HER2 antibody, displaying excellent dispersity in various aqueous solutions. This unique combination of nanoparticles and ligand molecules allows us to perform photothermal treatment (PTT) of the cancer cells, while simultaneously quantifying the distribution of MFNPs on a cancer cell surface induced by antigen antibody binding events. An important finding is that cancer cells adjacent to each other or in physical proximity within micrometers may end up with different fates of survival or death in PTT. This dramatic difference is determined by the antigen antibody binding events at the interface of MFNPs and cells because of tumor cell heterogeneity. Therefore, our experiments reveal a new scale of the highly localized feature of the photothermal effect at the single cell level illuminated by a continuous wave near IR laser.