Biosynthesis of poly(hydroxybutyrate-hydroxyvalerate) from the acclimated activated sludge and microbial characterization in this process.
Biosynthesis of poly(hydroxybutyrate-hydroxyvalerate) from the acclimated activated sludge and microbial characterization in this process.
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DOI：
10.1016/j.biortech.2013.08.102
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发表时间：
2013-11
影响因子：
11.4
通讯作者：
Yuanpeng Wang;J. Cai;Jianghuang Lan;Zhenggui Liu;N. He;Liang Shen;Qingbiao Li
中科院分区：
文献类型：
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作者：
Yuanpeng Wang;J. Cai;Jianghuang Lan;Zhenggui Liu;N. He;Liang Shen;Qingbiao Li
采用单一碳源(醋酸酯)和混合碳源(醋酸酯和丙酸)驯化活性污泥，考察了底物组成(醋酸酯和丙酸)对合成不同比例的聚羟基丁酸酯-羟基戊酸酯(P(HB/HV))的影响。分批生产P(HB/HV)的结果表明，随着底物中丙酸比例的增加，P(HB/HV)的产率和合成速率降低。然而，在底物利用率、P(HB/HV)产量和P(HB/HV)中HV含量方面，混合碳驯化污泥与醋酸盐驯化污泥相比，表现出更好的产磷性能。根据底物组成和污泥驯化条件，可以得到所需的羟基戊酸酯(HV)分数(0-74%)。此外，PCR-DGGE分析表明，用于生产P(HB/HV)的活性污泥以特定种类为主。酸杆菌和伯克霍尔德菌是优势菌群，在HV合成中起着重要作用。
This study investigated the effects of substrate composition (acetate and propionate) on synthesis of various mix of poly(hydroxybutyrate-hydroxyvalerate) (P(HB/HV)) from activated sludge, which was acclimated using a single carbon (acetate) and mixed carbons (acetate and propionate). Results of batch P(HB/HV) production tests indicated that the yield and synthesis rate of P(HB/HV) decreased as the proportion of propionate in the substrate increased. However, mixed-carbon-acclimated sludge with acetate and propionate exhibited better P(HB/HV) production performance than with acetate-acclimated sludge in terms of substrate utilization, yield of P(HB/HV) and HV fraction in P(HB/HV). The desired hydroxyvalerate (HV) fraction (0–74%) of the P(HB/HV) could be obtained based on the substrate composition and sludge acclimation. Furthermore, PCR–DGGE analysis indicated that specific species dominated the activated sludge used for P(HB/HV) production. Acidobacteria and Burkholderiales were the dominant bacterial populations and played an important role in HV synthesis.