DELLA-mediated PIF degradation contributes to coordination of light and gibberellin signalling in Arabidopsis.
DELLA-mediated PIF degradation contributes to coordination of light and gibberellin signalling in Arabidopsis.
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DELLA 介导的 PIF 降解有助于拟南芥中光和赤霉素信号传导的协调
DOI：
10.1038/ncomms11868
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发表时间：
2016-06-10
影响因子：
16.6
通讯作者：
Deng XW
中科院分区：
文献类型：
--
作者：
Li K;Yu R;Fan LM;Wei N;Chen H;Deng XW
光和赤霉素（GAs）拮抗调节植物下胚轴伸长。研究表明，作为GA信号的负调节因子，DELLAs通过隔离光敏色素相互作用因子3和4 （PIF3和PIF4）的dna识别结构域来抑制它们。然而，目前尚不清楚在DELLAs和pif之间是否存在其他的调控机制。在这里，我们证明DELLAs通过泛素-蛋白酶体系统负向调节四种PIF蛋白的丰度。DELLAs降低PIF3蛋白丰度与降低下胚轴伸长密切相关。DELLAs对PIF3的隔离和降解都有助于减少PIF3与其靶基因的结合。因此，我们表明DELLAs促进PIF降解是协调光和GA信号所必需的，DELLAs通过固存和降解对转录因子的双重调节可能是一种普遍的机制。
Light and gibberellins (GAs) antagonistically regulate hypocotyl elongation in plants. It has been demonstrated that DELLAs, which are negative regulators of GA signalling, inhibit phytochrome-interacting factors 3 and 4 (PIF3 and PIF4) by sequestering their DNA-recognition domains. However, it is unclear whether there are other mechanisms of regulatory crosstalk between DELLAs and PIFs. Here, we demonstrate that DELLAs negatively regulate the abundance of four PIF proteins through the ubiquitin–proteasome system. Reduction of PIF3 protein abundance by DELLAs correlates closely with reduced hypocotyl elongation. Both sequestration and degradation of PIF3 by DELLAs contribute to a reduction in PIF3 binding to its target genes. Thus, we show that promotion of PIF degradation by DELLAs is required to coordinate light and GA signals, and the dual regulation of transcription factors by DELLAs by both sequestration and degradation may be a general mechanism.