UNIQUE SEQUENCE, SKI, IN SLOAN-KETTERING AVIAN RETROVIRUSES WITH PROPERTIES OF A NEW CELL-DERIVED ONCOGENE
UNIQUE SEQUENCE, SKI, IN SLOAN-KETTERING AVIAN RETROVIRUSES WITH PROPERTIES OF A NEW CELL-DERIVED ONCOGENE
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DOI：
10.1128/jvi.57.3.1065-1072.1986
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发表时间：
1986-03-01
影响因子：
5.4
通讯作者：
STAVNEZER, E
中科院分区：
文献类型：
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作者：
LI, Y;TURCK, CM;STAVNEZER, E
斯隆-凯特林病毒(SKV)是从禽白血病病毒转化缺陷布拉迪斯拉发77(TdB77)感染的鸡胚胎细胞中分离到的一组转化逆转录病毒。每个SKV分离株都被证明含有多个大小不同的基因组，这表明除了tdB77外还存在几种病毒。为了鉴定和鉴定SKV的可能转化基因(S)，我们采用杂交选择的方法分离了SKV特异的代表基因片段。与病毒RNA的溶液和斑点杂交研究表明，该特异探针包含一个序列SKI，该序列至少部分被多个SKV基因组所共有。观察到一个分子克隆的SKI探针也与多个SKV基因组中的每一个杂交，证实了这一结论。鸡DNA的Southern杂交结果显示，鸡的SKI(c-ski)基因与内源病毒位点无关。结果表明，c-ski在未感染鸡细胞的多聚腺化胞质RNA中有表达，表明c-ski是一个功能基因。其他数据显示，c-ski在鸟类和哺乳动物的进化中发生了逆转，这表明该基因在鸡以外的物种中发挥了功能作用。用SKI基因在溶液中与病毒RNA杂交，在Southern杂交和克隆的逆转录病毒癌基因中，我们没有发现SKI与先前发现的15个癌基因中的任何一个有任何关系。
The Sloan-Kettering viruses (SKVs) are a group of transforming retroviruses that were isolated from chicken embryo cells which had been infected with the avian leukosis virus transformation-defective Bratislava 77 (tdB77). Each of the SKV isolates was shown to contain multiple genomes of different sizes indicating the presence of several viruses in addition to tdB77. To identify and characterize the putative transforming gene(s) of the SKVs, we used hybridization selection to isolate the fraction of a representative cDNA which was SKV specific. Both solution and blot hybridization studies with viral RNAs showed that the specific probe contained a sequence ski, that was at least partially held in common by the multiple SKV genomes. This conclusion was confirmed by the observation that a molecularly cloned ski probe also hybridized to each of the multiple SKV genomes. Southern blots of chicken DNA revealed homologs of ski (c-ski) which were not associated with endogenous viral loci. Results showing that c-ski was expressed in polyadenylated cytoplasmic RNA of uninfected chicken cells indicated that it is a functional gene. Other data showed that c-ski was conversed in avian and mammalian evolution, suggesting a functional role of the gene in species other than chickens. Using ski cDNA in solution hybridizations with viral RNAs and in Southern blot hybridization and cloned retroviral oncogenes, we did not detect any relationship between ski and any of 15 previously identified oncogenes.