Efficient delivery of antigen to DCs using yeast-derived microparticles.
Efficient delivery of antigen to DCs using yeast-derived microparticles.
复制标题
使用酵母来源的微粒将抗原有效递送至 DC
DOI：
10.1038/srep10687
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发表时间：
2015-05-29
影响因子：
4.6
通讯作者：
Gou M
中科院分区：
文献类型：
--
作者：
Pan Y;Li X;Kang T;Meng H;Chen Z;Yang L;Wu Y;Wei Y;Gou M
一些病原体可以在体内被抗原递呈细胞（APC）自然识别和内化，为高效疫苗递送提供平台。然而，生物安全问题阻碍了活病原体的临床应用。在此，制备酵母衍生的微粒用于癌症疫苗递送。通过化学处理面包酵母，获得了荚膜酵母壳（YS）微粒。将卵清蛋白（OVA）作为模型抗原偶联到YS的表面。结果表明，这些YS微粒大小均一，约为3.4 μm，可被树突状细胞（DCs）识别并内化。YS介导的抗原递送可以增强DC对抗原的细胞摄取，促进DC的成熟，并触发DC释放免疫共刺激分子。YS介导的抗原免疫可诱导机体产生有效的抗肿瘤免疫应答，其中体液免疫和细胞免疫共同发挥作用。本工作表明酵母壳微粒作为一种有效的疫苗载体系统在肿瘤免疫治疗中具有广阔的应用前景。
Some pathogens can be naturally recognized and internalized by antigen presentation cells (APCs)in vivo, providing a platform for efficient vaccine delivery. However, the biosafety concerns discourage the clinical applications of live pathogens. Here, yeast-derived microparticles were prepared for cancer vaccine delivery. By chemical treatment of bread yeast, capsular yeast shell (YS) microparticles were obtained. Ovalbumin (OVA), as a model antigen, was conjugated to the surface of YS. Results indicated that these YS microparticles with a uniform size of ~3.4 μm can be recognized and internalized by dendritic cells (DCs). The YS-mediated antigen delivery can enhance the cellular uptake of antigen by DCs, promote the maturation of DCs and trigger DCs to release immune co-stimulatory molecules. Immunization with YS-mediated antigen can induce an effective immune response against tumor cellsin vivo, with contributions from both humoral and cellular immunity. This work suggests that yeast shell microparticles as efficient vaccine delivery system has promising applications in cancer immunotherapy.