Human CRYL1 a novel enzyme-crystallin overexpressed in liver and kidney and downregulated in 58% of liver cancer tissues from 60 Chinese patients, and four new homologs from other mammalians
Human CRYL1 a novel enzyme-crystallin overexpressed in liver and kidney and downregulated in 58% of liver cancer tissues from 60 Chinese patients, and four new homologs from other mammalians
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DOI：
10.1016/s0378-1119(02)01095-8
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发表时间：
2003-01-02
期刊：
影响因子：
3.5
通讯作者：
Zhao, SY
中科院分区：
文献类型：
--
作者：
Chen, J;Yu, L;Zhao, SY
λ-晶状体蛋白是兔和野兔透镜的一种成分。它含有推测的NAD或FAD结合结构域，在3-羟酰辅酶A脱氢酶中被命名为HCDH结构域。在我们试图寻找肝癌组织和正常组织之间差异表达的基因中，鉴定了人晶状体蛋白1（crystallin，λ 1）。在60例中国人肝癌组织中，58%的肝癌组织表达下调。推测的蛋白质编码的MACL 1股83%的同源性与兔的晶状体蛋白，并含有两个HCDH结构域。有趣的是，在肝脏和肾脏中，CD 3L 1 mRNA水平非常高，而在外周血白细胞和胸腺中，CD 3L 1 mRNA水平极低。在肝脏和肾脏中的表达量分别是其他14种组织中表达量的1.6倍和1.2倍。这种特殊的表达模式和推测的HCDH结构表明，该蛋白可能具有与3-羟酰辅酶A脱氢酶类似的功能。为了进一步了解晶状体蛋白家族，我们从小鼠、大鼠、牛和猪中克隆了四个新的哺乳动物同源物。绘制了该蛋白家族包括人类和其他26种物种的无根系统发育树，分析了它们之间的进化关系。此外，人CryL 1定位于染色体13q12.11，小鼠Cry 11定位于染色体14的标记D14 Mit 83和D14 Mit 260之间。(C)2002 Elsevier Science B. V.保留所有权利。
Lambda-crystallin is a composition of lens in rabbit and hare. It contains the putative NAD- or FAD-binding domain, which is named as HCDH domain in 3-hydroxyacyl-CoA dehydrogenase. In our attempt to search for genes differentially expressed between liver cancer tissues and normal tissues, human CRYL1 (crystallin, lambda 1) was identified. It was downregulated in 58% of 60 Chinese HCC tissue samples. The putative protein encoded by CRYL1 shares 83% identity with rabbit lambda-crystallin and contains two HCDH domains. Interestingly, CRYL1 mRNA level is remarkably high in liver and kidney, while it is extremely low in peripheral blood leukocyte and thymus. The CRYL1mRNA levels in liver and kidney are about 1.6 and 1.2 times the total amount of that in other 14 tissues, respectively. Both the special expression pattern and the putative HCDH structure of CRYL1 suggested that the protein may be of the similar function of 3-hydroxyacyl-CoA dehydrogenase. To further understand the lambda-crystallin protein family, we cloned four novel mammalian homologs from mouse, rat, bovine and pig. The unrooted phylogenetic tree of this protein family including human and other 26 species was drawn to analyse their evolutionary relationship. In addition, human CRYL1 was mapped to chromosome 13q12.11 and mouse Cryl1 to chromosome 14 between marker D14Mit83 and D14Mit260. (C) 2002 Elsevier Science B.V. All rights reserved.