Selective alkylation of poly(A) tracts of RNA inside the cell with the derivative of ethyl ester of oligothymidilate bearing 2‐Chloroethylamino group
Selective alkylation of poly(A) tracts of RNA inside the cell with the derivative of ethyl ester of oligothymidilate bearing 2‐Chloroethylamino group
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使用带有 2-氯乙基氨基的寡胸苷酸乙酯衍生物对细胞内 RNA 的聚 (A) 束进行选择性烷基化
DOI：
10.1016/0014-5793(80)80392-9
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发表时间：
1980
期刊：
影响因子：
3.5
通讯作者：
L. E. Stephanovich
中科院分区：
文献类型：
--
作者：
G. Karpova;D. Knorre;A. Ryte;L. E. Stephanovich
互补寻址修饰方法由NI Grineva等人提出[1]并实现[2-41]，在预定点对单链核酸进行选择性标记。阐述了将活性的2-氯乙胺基团与寡核苷酸的3′或5′端结合的方法，将试剂导向核酸的选定区域[5,6]。根据鸟嘌呤残基[7]上的2-氯乙胺基mRNA衍生物，描述了T7 DNA的选择性诱变。1990年也提出了类似的想法。
The method of the complementary addressed modification was proposed [I] and realised [2-41 by NI Grineva et al. to perform the selective labeling of single-stranded nucleic acids at the predetermined points. The methods were elaborated for the binding of the reactive 2-chloroethylamino group to either 3’-or 5’-end of oligonucleotides directing the reagent to the chosen region of nucleic acid [5, 6]. The selective mutagenesis of T7 DNA has been described, based on the mRNA derivatives with 2-chloroethylamino groups attached to the guanine residues [7]. A similar idea was presented in [8].