Nanofluidic redox cycling amplification for the selective detection of catechol
Nanofluidic redox cycling amplification for the selective detection of catechol
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DOI：
10.1021/ac7016647
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发表时间：
2008-02-15
影响因子：
7.4
通讯作者：
Lemay, Serge
中科院分区：
文献类型：
--
作者：
Wolfrum, Bernhard;Zevenbergen, Marcel;Lemay, Serge
我们已经开发了一种基于芯片的纳米流体装置，以放大邻苯二酚的电化学信号的数量级。放大是基于纳米通道内的平面平行电极之间的快速氧化还原循环。我们表明，它是可能的，以监测只有几百个分子居住在纳米流体传感器的活性区域的信号。此外，由于纳米通道设计，传感器不受经历不可逆氧化还原反应的分子的干扰。我们证明了设备的选择性，通过检测邻苯二酚的存在下，抗坏血酸，其氧化形式是唯一的。短时间内保持稳定。抗坏血酸的干扰是生物样品中儿茶酚胺检测的一个难题。
We have developed a chip-based nanofluidic device to amplify the electrochemical signal of catechols by orders of magnitude. The amplification is based on rapid redox cycling between plane parallel electrodes inside a nanochannel. We show that it is possible to monitor the signal of only a few hundred molecules residing in the active area of the nanofluidic sensor. Furthermore, due to the nanochannel design, the sensor is immune to interference by molecules undergoing irreversible redox reactions. We demonstrate the selectivity of the device by detecting catechol in the presence of ascorbic acid, whose oxidized form is only. stable for a short time. The interference of ascorbic acid is usually a challenge in the detection of catecholamines in biological samples.