High genetic diversity and adaptive potential of two simian hemorrhagic fever viruses in a wild primate population.
High genetic diversity and adaptive potential of two simian hemorrhagic fever viruses in a wild primate population.
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DOI：
10.1371/journal.pone.0090714
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
O'Connor DH
中科院分区：
文献类型：
--
作者：
Bailey AL;Lauck M;Weiler A;Sibley SD;Dinis JM;Bergman Z;Nelson CW;Correll M;Gleicher M;Hyeroba D;Tumukunde A;Weny G;Chapman C;Kuhn JH;Hughes AL;Friedrich TC;Goldberg TL;O'Connor DH
高遗传多样性和高进化速率等关键生物学特性增强了某些RNA病毒适应和出现的潜力。在其自然宿主中识别具有这些特性的病毒可以大大改善疾病预测和监测。最近，我们发现了两个新成员的病毒家族动脉炎病毒：猴出血热病毒（SHFV）-krc 1和SHFV-krc 2，感染一个单一的野生红疣猴（红疣猴tephrosceles）在基巴莱国家公园，乌干达。关于自然界中SHFV的生物学特性几乎一无所知，尽管SHFV型毒株SHFV-LVR在圈养猕猴中引起了毁灭性的病毒性出血热爆发。在60只野生红疣猴中，分别有40%和47%的人检测到SHFV-krc 1和SHFV-krc 2。我们发现这些病毒的病毒载量超过每毫升血浆106-107个RNA拷贝。SHFV-krc 1和SHFV-krc 2在宿主间和宿主内均表现出较高的遗传多样性。对病毒基因组中同义和非同义核苷酸多样性的分析揭示了提示SHFV开放阅读框（ORF）5（仅SHFV-krc 2）和7（SHFV-krc 1和SHFV-krc 2）中正选择的模式。因此，这些病毒与一些进化最快的新出现的RNA病毒有几个重要的特性。
Key biological properties such as high genetic diversity and high evolutionary rate enhance the potential of certain RNA viruses to adapt and emerge. Identifying viruses with these properties in their natural hosts could dramatically improve disease forecasting and surveillance. Recently, we discovered two novel members of the viral family Arteriviridae: simian hemorrhagic fever virus (SHFV)-krc1 and SHFV-krc2, infecting a single wild red colobus (Procolobus rufomitratus tephrosceles) in Kibale National Park, Uganda. Nearly nothing is known about the biological properties of SHFVs in nature, although the SHFV type strain, SHFV-LVR, has caused devastating outbreaks of viral hemorrhagic fever in captive macaques. Here we detected SHFV-krc1 and SHFV-krc2 in 40% and 47% of 60 wild red colobus tested, respectively. We found viral loads in excess of 106–107 RNA copies per milliliter of blood plasma for each of these viruses. SHFV-krc1 and SHFV-krc2 also showed high genetic diversity at both the inter- and intra-host levels. Analyses of synonymous and non-synonymous nucleotide diversity across viral genomes revealed patterns suggestive of positive selection in SHFV open reading frames (ORF) 5 (SHFV-krc2 only) and 7 (SHFV-krc1 and SHFV-krc2). Thus, these viruses share several important properties with some of the most rapidly evolving, emergent RNA viruses.
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影响因子：
3.2
作者：
Chen, ZY;Li, K;Plagemann, PGW
通讯作者：
Plagemann, PGW
影响因子：
5.4
作者：
Ansari, IH;Kwon, B;Pattnaik, AK
通讯作者：
Pattnaik, AK
DOI：
10.1073/pnas.0802203105
发表时间：
2008-05-27
影响因子：
11.1
作者：
Keele, Brandon F.;Giorgi, Elena E.;Shaw, George M.
通讯作者：
Shaw, George M.
影响因子：
3.7
作者：
Hedges, JF;Balasuriya, UBR;MacLachlan, NJ
通讯作者：
MacLachlan, NJ
影响因子：
4.3
作者：
Holmes EC;Grenfell BT
通讯作者：
Grenfell BT