An ultrastructural study of mast cells in the alveolar wall of normal and asthmatic lung.
An ultrastructural study of mast cells in the alveolar wall of normal and asthmatic lung.
复制标题
正常和哮喘肺肺泡壁肥大细胞的超微结构研究。
DOI：
10.1038/icb.1986.46
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
J. Paterson
中科院分区：
文献类型：
--
作者：
A. Warton;J. Papadimitriou;R. Goldie;J. Paterson
电镜观察正常肺和哮喘肺标本，用形态计量学技术观察肺泡壁肥大细胞及其颗粒的频率和超微结构特征。正常肺每平方毫米肺泡壁肥大细胞密度299个（SD = 258），哮喘肺每平方毫米肺泡壁肥大细胞密度366个（SD = 260）。正常肺肥大细胞有核区平均面积为25.7微米s2 (SD = 6.3)，哮喘肺肥大细胞有核区平均面积为29.8微米s2 （SD = 6.2）。正常肺每单个肥大细胞有核谱平均分泌颗粒数为55粒（SD = 13），哮喘肺平均分泌颗粒数为60粒（SD = 12）。正常肺分泌颗粒直径338.9 nm (SD = 42.6)，哮喘肺分泌颗粒直径345.6 nm （SD = 47.7）。正常肺和哮喘肺分泌颗粒体积密度分别为6.31和5.81 μ s。在正常和哮喘肺中，分泌颗粒内单个亚基（“涡旋”）的平均直径为88.8 nm。在正常肺中，64.2%的颗粒为“卷状”和“混合型”颗粒，35.8%的颗粒为“颗粒”或“空”颗粒。在哮喘患者的样本中，40.3%的颗粒具有“卷轴”或“组合”结构，59.7%为“颗粒”或“空”结构。我们的数据表明，正常和哮喘肺肥大细胞的数量没有差异。然而，在哮喘肺肥大细胞中，脱颗粒比正常肺更常见。
Specimens of normal and asthmatic lungs were studied at the electron microscopic level and the frequency and ultrastructural features of mast cells and their granules within the alveolar wall were assessed with morphometric techniques. The numerical density of mast cells per square millimetre of alveolar wall was 299 (SD = 258) in normal and 366 (SD = 260) in asthmatic lung. The mean area of the mast cell nucleated profile was 25.7 microns2 (SD = 6.3) in normal lung and 29.8 (SD = 6.2) in asthmatic. The average number of secretory granules per single mast cell nucleated profile was 55 (SD = 13) in normal lung and 60 (SD = 12) in asthmatic lung. The diameter of the individual secretory granule was 338.9 nm (SD = 42.6) in normal and 345.6 (SD = 47.7) in asthmatic lung. The volume density of secretory granules in normal and asthmatic lung was 6.31 microns3 and 5.81 microns3, respectively. The mean diameter of the individual subunit ('scroll') inside the secretory granule was 88.8 nm for both normal and asthmatic lung. In normal lung 64.2% of granules were of 'scroll' and 'combined' type, and 35.8% of granules were 'particulate' or 'empty'. In specimens from asthmatic patients 40.3% of granules had 'scroll' or 'combined' structures and 59.7% were 'particulate' or 'empty'. Our data suggest that there is no difference between the number of mast cells in normal and asthmatic lung. However, in pulmonary mast cells from asthmatic lung, degranulation is more common than in normal lung.