Cerebrospinal Fluid Leak Detection with a Carbon Nanotube-Based Field-Effect Transistor Biosensing Platform
Cerebrospinal Fluid Leak Detection with a Carbon Nanotube-Based Field-Effect Transistor Biosensing Platform
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DOI：
10.1021/acsami.1c19120
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发表时间：
2021-12-21
影响因子：
9.5
通讯作者：
Star, Alexander
中科院分区：
文献类型：
--
作者：
Shao, Wenting;Shurin, Galina, V;Star, Alexander
脑脊液（CSF）渗漏如果不及时发现，可能导致危及生命的并发症。然而，凝胶电泳，通过检测β 2-转铁蛋白（β 2-Tf）确认CSF渗漏的金标准测试，需要3-6小时，而且是劳动密集型的。我们开发了一种新的β 2-Tf检测平台，用于快速鉴定CSF渗漏。三步设计，其中包括两个步骤的亲和层析和一个快速的传感步骤，使用一个生物富集的单壁碳纳米管场效应晶体管（FET）传感器，避免了缺乏选择性，抗转铁蛋白抗体表现出转铁蛋白亚型，并显着缩短了检测时间。此外，研究了FET传感器的三种不同感测配置以获得最佳β 2-TI感测结果。最后，采用我们的三步策略进行的体液（CSF和血清）检测显示出高灵敏度，表明其有可能用作CSF渗漏的快速诊断工具。
Cerebrospinal fluid (CSF) leakage may lead to life-threatening complications if not detected promptly. However, gel electrophoresis, the gold-standard test for confirming CSF leakage by detecting beta2-transferrin (beta 2-Tf), requires 3-6 h and is labor-intensive. We developed a new beta 2-Tf detection platform for rapid identification of CSF leakage. The three-step design, which includes two steps of affinity chromatography and a rapid sensing step using a semiconductor-enriched single-walled carbon nanotube field-effect transistor (FET) sensor, circumvented the lack of selectivity that antitransferrin antibody exhibits for transferrin isoforms and markedly shortened the detection time. Furthermore, three different sensing configurations for the FET sensor were investigated for obtaining the optimal beta 2-TI sensing results. Finally, body fluid (CSF and serum) tests employing our three-step strategy demonstrated high sensitivity, suggesting its potential to be used as a rapid diagnostic tool for CSF leakage.