Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice
Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice
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DOI：
10.1007/s00436-015-4737-z
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发表时间：
2016-01-01
影响因子：
2
通讯作者：
Matsuo, Tomohide
中科院分区：
文献类型：
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作者：
Ono, Yuina;Matsubayashi, Makoto;Matsuo, Tomohide
最近，我们展示了克氏艾美耳球虫作为一种新的小鼠艾美耳球虫寄生虫在阐明生物多样性方面的作用。该寄生虫对不同免疫状态和对包括球虫胺在内的抗菌剂敏感性不同的小鼠表现出显着的感染性。然而，krijgsmanni的详细生活史尚未确定，在讨论以前的调查结果时缺乏这方面的信息。本研究阐明了克氏艾美耳球虫的形态特征及其在正常小鼠中的生活周期，并检测了初次接种后对免疫缺陷小鼠和生命周期阶段的攻击感染的影响。在具有免疫能力的小鼠中，生命周期由四个无性阶段和有性鼠尾草组成，随后在潜伏期前形成卵囊。有趣的是，在其他阶段消失后的所有观察期中都检测到了第二代子粒。对于免疫缺陷小鼠的攻击感染，除第二代小鼠外，其余发育阶段均暂时消失。这一发现表明，发育的“休息”或明显延迟，以及对下一代的促进“重新开始”。Krijgsmanni的第二代分体可能在其生活史中起重要作用。
Recently, we have demonstrated the utility of Eimeria krijgsmanni as a novel mouse eimerian parasite for elucidating the biological diversity. The parasite showed notable infectivity to mice with various levels of immune status and susceptibility to antimicrobial agents including coccidiostat. However, the detailed lifecycle of E. krijgsmanni had not yet been determined and this information was lacking in discussion of previous findings. In the present study, we clarified the morphological characteristics of E. krijgsmanni and its lifecycle in normal mice, and examined the effects in immunodeficient mice and lifecycle stage for challenge infections after the primary inoculation. In immunocompetent mice, the lifecycle consisted of four asexual stages and the sexual sages followed by formation of oocysts during the prepatent periods. Interestingly, the second-generation meronts were detected in all observation periods after the disappearance of the other stages. For the challenge infection of immunodeficient mice, all developmental stages except for the second generation meronts were temporarily vanished. This finding suggests a "rest" or marked delay in development and a "restart" of the promotion toward the next generations. The second generation meronts may play an important role in the lifecycle of E. krijgsmanni.