Female-specific flightless phenotype for mosquito control
Female-specific flightless phenotype for mosquito control
复制标题
DOI：
10.1073/pnas.1000251107
复制
发表时间：
2010-03-09
影响因子：
11.1
通讯作者：
Alphey, Luke
中科院分区：
文献类型：
--
作者：
Fu, Guoliang;Lees, Rosemary S.;Alphey, Luke
登革热和登革出血热正在加剧公共卫生问题，估计每年有5000万至1亿新感染病例。埃及伊蚊是登革热病毒在其传播范围内的主要媒介，控制这种蚊子将显著降低人类发病率和死亡率。现有的防蚊方法效果不理想，急需新的防蚊方法。一种基于释放携带条件显性致死基因的蚊子的“不育雄性释放”策略是一种有吸引力的新控制方法。利用来自埃及伊蚊肌动蛋白-4基因的雌性特异性间接飞行肌肉启动子，利用两个单独的转基因或单个转基因，对埃及伊蚊转基因菌株进行了改造，使其具有可抑制的雌性特异性无飞行表型。这些菌株消除了辐照灭菌的需要，只允许雄性释放（“遗传性别”），并且可以释放卵子而不是成虫。此外，如果将这些菌株作为病虫害综合管理战略的一部分加以采用，预计将有助于在全地区控制或消除登革热。
Dengue and dengue hemorrhagic fever are increasing public health problems with an estimated 50-100 million new infections each year. Aedes aegypti is the major vector of dengue viruses in its range and control of this mosquito would reduce significantly human morbidity and mortality. Present mosquito control methods are not sufficiently effective and new approaches are needed urgently. A "sterile-male-release" strategy based on the release of mosquitoes carrying a conditional dominant lethal gene is an attractive new control methodology. Transgenic strains of Aedes aegypti were engineered to have a repressible female-specific flightless phenotype using either two separate transgenes or a single transgene, based on the use of a female-specific indirect flight muscle promoter from the Aedes aegypti Actin-4 gene. These strains eliminate the need for sterilization by irradiation, permit male-only release ("genetic sexing"), and enable the release of eggs instead of adults. Furthermore, these strains are expected to facilitate area-wide control or elimination of dengue if adopted as part of an integrated pest management strategy.