Di(n-butyl) phthalate exposure impairs meiotic competence and development of mouse oocyte
Di(n-butyl) phthalate exposure impairs meiotic competence and development of mouse oocyte
复制标题
邻苯二甲酸二正丁酯暴露会损害小鼠卵母细胞的减数分裂能力和发育
DOI：
10.1016/j.envpol.2018.12.077
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发表时间：
2019-03-01
影响因子：
8.9
通讯作者：
Wang, Hai-Long
中科院分区：
文献类型：
--
作者：
Li, Fei-Ping;Zhou, Jie-Long;Wang, Hai-Long
邻苯二甲酸二丁酯（DBP）作为增塑剂和稳定剂广泛应用于工业领域，其在环境中的存在可能对人类健康构成威胁。已有研究表明其具有致突变、致畸和致癌的能力。然而，其对哺乳动物卵母细胞成熟的影响仍然未知。在这项研究中，我们研究了DBP在体外和体内对卵母细胞成熟的影响。结果表明，DBP能显著降低小鼠卵母细胞萌发泡破裂率（GVBD）和极体排出率（PBE）。此外，卵母细胞的细胞骨架被破坏，皮质颗粒自由结构域（CGFD）也被破坏。DBP诱导卵母细胞和颗粒细胞凋亡。总的来说，这些数据表明DBP可以降低小鼠卵母细胞减数分裂能力和发育。(C)2018由Elsevier Ltd.出版
Di(n-butyl) phthalate (DBP) is extensively used in industrial applications as plasticizer and stabilizer and its presence in the environment may present health risks for human. Previous studies have demonstrated its mutagenic, teratogenic, and carcinogenic ability. However, its effect on mammalian oocyte maturation remains unknown. In this study, we examined the effect of DBP on oocyte maturation both in vitro and in vivo. Our results showed that DBP could significantly reduce mice oocyte germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) rates. In addition, oocyte cytoskeleton was damaged and cortical granule-free domains (CGFDs) were also disrupted. Finally, DBP induced early apoptosis of oocyte and granulosa cells (GCs). Collectively, these data demonstrate that DBP could reduce meiosis competence and mouse oocyte development. (C) 2018 Published by Elsevier Ltd.