N-hydroxy-pipecolic acid is a mobile metabolite that induces systemic disease resistance in Arabidopsis
N-hydroxy-pipecolic acid is a mobile metabolite that induces systemic disease resistance in Arabidopsis
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DOI：
10.1073/pnas.1805291115
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发表时间：
2018-05-22
影响因子：
11.1
通讯作者：
Sattely, Elizabeth S.
中科院分区：
文献类型：
--
作者：
Chen, Yun-Chu;Holmes, Eric C.;Sattely, Elizabeth S.
系统获得性抗性（systemic acquired resistance，SAR）是植物在感染部位诱导的一种全局性反应，它能在远端未感染组织产生持久的广谱抗病性。尽管这种启动机制的重要性，启动SAR信号所需的防御信号的身份和复杂性还没有得到很好的理解。在本文中，我们描述了一种代谢产物，N-羟基-哌啶酸（N-OH-Pip），并提供证据表明，这个移动的分子在启动SAR信号转导在拟南芥中发挥作用。我们证明，黄素依赖性单加氧酶1（FMO 1），SAR相关的防御启动的关键调节剂，可以合成N-OH-Pip从哌啶酸在植物中，和外源施加的N-OH-Pip在拟南芥中系统移动，并可以拯救SAR缺陷的fmo 1突变体。我们还表明，N-OH-Pip治疗引起系统性的变化，在整个植物的发病相关基因和代谢途径的表达，并提高对细菌病原体的抗性。这项工作提供了深入了解SAR信号的化学性质，也表明N-OH-Pip途径是代谢工程提高抗病性的一个有前途的靶点。
Systemic acquired resistance (SAR) is a global response in plants induced at the site of infection that leads to long-lasting and broad-spectrum disease resistance at distal, uninfected tissues. Despite the importance of this priming mechanism, the identity and complexity of defense signals that are required to initiate SAR signaling is not well understood. In this paper, we describe a metabolite, N-hydroxy-pipecolic acid (N-OH-Pip) and provide evidence that this mobile molecule plays a role in initiating SAR signal transduction in Arabidopsis thaliana. We demonstrate that FLAVIN-DEPENDENT MONOOXYGENASE 1 (FMO1), a key regulator of SAR-associated defense priming, can synthesize N-OH-Pip from pipecolic acid in planta, and exogenously applied N-OH-Pip moves systemically in Arabidopsis and can rescue the SAR-deficiency of fmo1 mutants. We also demonstrate that N-OH-Pip treatment causes systemic changes in the expression of pathogenesis-related genes and metabolic pathways throughout the plant and enhances resistance to a bacterial pathogen. This work provides insight into the chemical nature of a signal for SAR and also suggests that the N-OH-Pip pathway is a promising target for metabolic engineering to enhance disease resistance.