TROPHOBLAST CELL-DIFFERENTIATION - ESTABLISHMENT, CHARACTERIZATION, AND MODULATION OF A RAT TROPHOBLAST CELL-LINE EXPRESSING MEMBERS OF THE PLACENTAL PROLACTIN FAMILY
TROPHOBLAST CELL-DIFFERENTIATION - ESTABLISHMENT, CHARACTERIZATION, AND MODULATION OF A RAT TROPHOBLAST CELL-LINE EXPRESSING MEMBERS OF THE PLACENTAL PROLACTIN FAMILY
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DOI：
10.1210/endo-129-6-2895
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发表时间：
1991-12-01
期刊：
影响因子：
4.8
通讯作者：
SOARES, MJ
中科院分区：
文献类型：
--
作者：
FARIA, TN;SOARES, MJ
本研究的目的是建立和表征来自大鼠绒毛膜癌的细胞系，并评估该细胞系作为研究滋养层细胞分化的体外模型的有用性。从绒毛膜癌外植体产生细胞系并命名为Rcho-1。该细胞系由细胞类型的混合物组成，包括成簇生长的小细胞和具有非常大的细胞核的巨细胞。这种特征性的形态保持通过至少23代，并在一系列的克隆细胞系分离的亲本Rcho-1细胞系。Rcho-1细胞系在体外培养时能够表达胎盘催乳素-I（PL-I）、PL-II、PRL样蛋白-A（PLP-A）和PLP-C mRNA;然而，Rcho-1细胞在宿主大鼠肾包膜下生长时仅表达PL-I。Rcho-1细胞系在任何实验条件下均不表达PLP-B。这种胎盘PRL表达模式维持了23代。Rcho-1细胞合成和分泌PL-I，PL-II和PLP-A蛋白，其生化特性与胎盘对应物相似。PL-Ⅰ和PL-Ⅱ mRNA特异性定位于巨细胞，以形态学和胎盘PRL表达为指标监测不同条件下Rcho-1细胞的分化状态。形态和功能的滋养层细胞分化诱导维持Rcho-1细胞在汇合后培养条件。汇合后的Rcho-1培养物的特征在于巨细胞百分比的增加和胎盘PRL表达的诱导。从亲本Rcho-1细胞系衍生的一些克隆细胞系表现出不同的分化模式和胎盘PRL表达。总之，我们建立了一个能够表达分化表型的大鼠滋养层细胞系。分化的表型包括形态和功能参数，并且可以在体外调节。该细胞系是研究胎盘PRL基因表达控制和滋养层细胞分化调节的独特模型。
The purpose of this investigation was to establish and characterize a cell line derived from a rat choriocarcinoma and to evaluate the usefulness of the cell line as an in vitro model for studying trophoblast cell differentiation. A cell line was generated from choriocarcinoma explants and named Rcho-1. The cell line consisted of a mixture of cell types, including small cells growing in clusters and giant cells possessing very large nuclei. This characteristic morphology was maintained through at least 23 passages and in a series of clonal cell lines isolated from the parent Rcho-1 cell line. The Rcho-1 cell line was capable of expressing placental lactogen-I (PL-I), PL-II, PRL-like protein-A (PLP-A), and PLP-C mRNAs when cultivated in vitro; however, the Rcho-1 cells expressed only PL-I when grown beneath the kidney capsule of host rats. The Rcho-1 cell line did not express PLP-B under any experimental condition. This pattern of placental PRL expression was maintained for 23 passages. Rcho-1 cells synthesized and secreted PL-I, PL-II, and PLP-A proteins with biochemical characteristics similar to those of their placental counterparts. PL-I and PL-II mRNAs were specifically localized to giant cells.Morphological appearance and placental PRL expression were used as indices for monitoring the differentiation state of Rcho-1 cells grown under various conditions. Both morphological and functional trophoblast cell differentiation were induced by maintaining the Rcho-1 cells in postconfluent culture conditions. Postconfluent Rcho-1 cultures were characterized by an increased percentage of giant cells and an induction of placental PRL expression. Some clonal cell lines derived from the parent Rcho-1 cell line exhibited distinct patterns of differentiation and placental PRL expression.In summary, we have established a rat trophoblast cell line capable of expressing a differentiated phenotype. The differentiated phenotype includes both morphological and functional parameters and can be modulated in vitro. This cell line is a unique model for studying the control of placental PRL gene expression and the regulation of trophoblast cell differentiation.