“Potassium-induced cortical spreading depression bilaterally suppresses the electroencephalogram but only ipsilaterally affects red blood cell velocity in intraparenchymal capillaries.”
“Potassium-induced cortical spreading depression bilaterally suppresses the electroencephalogram but only ipsilaterally affects red blood cell velocity in intraparenchymal capillaries.”
复制标题
“钾诱导的皮质扩散抑制双侧抑制脑电图，但仅影响同侧实质内毛细血管中的红细胞速度。”
DOI：
10.1002/jnr.23184
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Suzuki N.
中科院分区：
文献类型：
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作者：
Unekawa M;Tomita Y;Toriumi H;Masamoto K;Kanno I;Suzuki N.
皮层扩散性抑制（CSD）是一种重复的，传播的神经元和神经胶质细胞的大量去极化，随后持续抑制自发神经元活动的轮廓。我们已经报告了对实质内毛细血管中红细胞（RBC）速度的长期抑制作用。在这里，为了检验毛细血管中RBC速度的长期降低是由于神经元活动受到抑制的假设，我们测量了CSD引起的脑电图（EEG）变化，作为神经元活动的指标。在异氟烷麻醉大鼠中，同时记录颞顶叶区的DC电位、EEG、氧分压（PO 2）和脑血流量（CBF）。使用我们的原始软件KEIO-IS 2通过高速相机激光扫描共聚焦荧光显微镜评估荧光标记的RBC的速度。局部应用KCl后，仅在同侧半球反复检测到DC电位和PO 2的瞬时偏转以及CBF的增加。另一方面，EEG的相对频谱功率在双侧降低，在施加KCl后5 min显示出最低值，此时其他参数在CSD通过后已恢复至基线。在同侧半球CSD通过期间和之后，毛细血管中的平均RBC速度轻微但显著降低，但在同一大鼠的对侧半球中没有变化。我们认为，大规模去极化的神经元和神经胶质细胞可能会暂时减速红细胞在附近的毛细血管，但持续减少同侧红细胞的速度可能是CSD的长期影响的结果，而不是单独的神经元抑制。© 2013 Wiley Periodicals，Inc.
Cortical spreading depression (CSD) is a repetitive, propagating profile of mass depolarization of neuronal and glial cells, followed by sustained suppression of spontaneous neuronal activity. We have reported a long‐lasting suppressive effect on red blood cell (RBC) velocities in intraparenchymal capillaries. Here, to test the hypothesis that the prolonged decrease of RBC velocity in capillaries is due to suppression of neuronal activity, we measured CSD‐elicited changes in the electroencephalogram (EEG) as an index of neuronal activity. In isoflurane‐anesthetized rats, DC potential, EEG, partial pressure of oxygen (PO2), and cerebral blood flow (CBF) were simultaneously recorded in the temporo‐parietal region. The velocities of fluorescently labeled RBCs were evaluated by high‐speed camera laser scanning confocal fluorescence microscopy with our original software, KEIO‐IS2. Transient deflection of DC potential and PO2and increase of CBF were repeatedly detected only in the ipsilateral hemisphere following topical KCl application. On the other hand, the relative spectral power of EEG was reduced bilaterally, showing the lowest value at 5 min after KCl application, when the other parameters had already returned to the baseline after the passage of CSD. Mean RBC velocity in capillaries was slightly but significantly reduced during and after passage of CSD in the ipsilateral hemisphere but did not change in the contralateral hemisphere in the same rats. We suggest that mass depolarization of neuronal and glial cells might transiently decelerate RBCs in nearby capillaries, but the sustained reduction of ipsilateral RBC velocity might be a result of the prolonged effect of CSD, not of neuronal suppression alone. © 2013 Wiley Periodicals, Inc.