A cryptic natural variant allele of BYPASS2 suppresses the bypass1 mutant phenotype
A cryptic natural variant allele of BYPASS2 suppresses the bypass1 mutant phenotype
复制标题
BYPASS2 的神秘自然变体等位基因抑制旁路 1 突变表型
DOI：
10.1093/plphys/kiad124
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发表时间：
2023
期刊：
影响因子：
7.4
通讯作者：
Sieburth, Leslie E.
中科院分区：
文献类型：
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作者：
Cummins, Alexander J.;Siler, C. J.;Olson, Jacob M.;Kaur, Amanpreet;Hamdani, Adam K.;Olson, L. Kate;Dilkes, Brian P.;Sieburth, Leslie E.
拟南芥BYPASS1(BPS1)基因编码一个没有功能结构域的蛋白质，功能缺失突变体(如Col-0中的bps1-2)表现出严重的生长停滞表型，这种生长停滞是由根来源的嫁接传递的小分子引起的，我们称之为dalekin。Dalekin信号从根到冠的本质表明，它可能是一种内源信号分子。在这里，我们报告了一个自然的变异筛选，它允许我们识别bps1-2突变表型的增强子和抑制子(在Col-0中)。我们在Apost-1的加入中发现了一个强大的半显性抑制基因，它在很大程度上恢复了bps 1的新梢发育，但仍继续过度生产达利金。通过批量分离分析和等位基因特异性的转基因互补分析，我们证明该抑制基因是BPS1 Paralog，BYPASS2(BPS2)的Apost-1等位基因。BPS2是拟南芥BPS基因家族的四个成员之一，系统发育分析表明BPS家族在陆地植物中是保守的，四个拟南芥基因家族在全基因组复制中保持重复。BPS1和Paralogous蛋白在整个陆地植物中的高度保守性，以及Paralog在拟南芥中类似的功能，表明dalekin信号可能在陆地植物中保留。
The Arabidopsis (Arabidopsis thaliana)BYPASS1(BPS1) gene encodes a protein with no functionally characterized domains, and loss-of-function mutants (e.g.bps1-2in Col-0) present a severe growth arrest phenotype that is evoked by a root-derived graft-transmissible small molecule that we call dalekin. The root-to-shoot nature of dalekin signaling suggests it could be an endogenous signaling molecule. Here, we report a natural variant screen that allowed us to identify enhancers and suppressors of thebps1-2mutant phenotype (in Col-0). We identified a strong semi-dominant suppressor in the Apost-1 accession that largely restored shoot development inbps1and yet continued to overproduce dalekin. Using bulked segregant analysis and allele-specific transgenic complementation, we showed that the suppressor is the Apost-1 allele of aBPS1paralog,BYPASS2(BPS2).BPS2is one of four members of theBPSgene family in Arabidopsis, and phylogenetic analysis demonstrated that theBPSfamily is conserved in land plants and the fourArabidopsisparalogs are retained duplicates from whole genome duplications. The strong conservation ofBPS1and paralogous proteins throughout land plants, and the similar functions of paralogs in Arabidopsis, suggests that dalekin signaling might be retained across land plants.