Mycoplasma bovis induces apoptosis of bovine lymphocytes
Mycoplasma bovis induces apoptosis of bovine lymphocytes
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DOI：
10.1111/j.1574-695x.2002.tb00540.x
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发表时间：
2002-01-14
影响因子：
--
通讯作者：
Rosenbusch, RF
中科院分区：
文献类型：
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作者：
Vanden Bush, TJ;Rosenbusch, RF
我们报告牛支原体诱导牛淋巴细胞凋亡。使用流式细胞术分析碘化丙啶内含物，我们观察到新鲜分离的牛PBMC与M.牛使用膜联蛋白V染色以及TUNEL检测证实了这些发现。此外，这些试验表明，M.牛诱导的淋巴细胞死亡本质上是凋亡。随后的实验表明，原核蛋白产生抑制剂氯霉素抑制M. bovis，表明M.牛蛋白的产生是诱导淋巴细胞死亡所必需的，并且该死亡不依赖于细胞凋亡诱导剂的加入，如其它支原体所示。我们还证明了M.牛支原体与其它牛支原体（致病性和非致病性）在这一特性方面不同。CC 2002欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
We report Mycoplasma bovis induces apoptotic death of bovine lymphocytes. Using flow cytometry analyzed propidium iodide inclusion we observed a loss in viable lymphocytes upon incubation of freshly isolated bovine PBMCs with M. bovis. The use of annexin V staining as well as TUNEL assays corroborated these findings. In addition, these assays indicated that the M. bovis induced lymphocyte death is apoptotic in nature. Subsequent experiments demonstrated that the prokaryotic protein production inhibitor chloramphenicol inhibited lymphocyte death induced by M. bovis, showing that M. bovis protein production is necessary for the induction of lymphocyte death, and that the death is not dependent upon the addition of apoptotic inducers as shown with other mycoplasmas. We also show that M. bovis is different from other bovine mycoplasmas (both pathogenic and non-pathogenic) with regards to this characteristic. CC 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.