Detection and genetic characterization of group a rotavirus strains circulating among children with acute gastroenteritis in Japan
Detection and genetic characterization of group a rotavirus strains circulating among children with acute gastroenteritis in Japan
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DOI：
10.1128/jvi.02342-06
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发表时间：
2007-05-01
影响因子：
5.4
通讯作者：
Ushijima, Hiroshi
中科院分区：
文献类型：
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作者：
Phan, Tung Gia;Khamrin, Pattara;Ushijima, Hiroshi
2005年7月至2006年6月，从日本5个不同地区的急性肠胃炎儿童中收集了603份粪便标本，采用逆转录-聚合酶链反应筛查A组轮状病毒。结果发现117份粪便标本（19.4%）检出A组轮状病毒阳性。11月至6月连续检测到轮状病毒感染，4月最高。进一步研究了G （VP7基因型）和P （VP4基因型）。主要基因型为G1P[8](70.1%)，其次为G3P[8] （17.90/c）、G9P[8]（6.8%）和G2P[4]（2.6%）。在此研究期间，还检测到许多不寻常的G1P[4]组合。提出了一种新的G1命名法，其中世界范围内的轮状病毒G1株分为11个谱系和17个亚谱系。在VP7中发现了广泛的氨基酸替换（多达34个），这些氨基酸替换是G1谱系和亚谱系特有的。有趣的是，只有位于VP7的第29 - 75位和211 - 213位的短氨基酸基序才能正确地定义G1谱系和亚谱系。对VP7抗原区序列的分析还发现了与系统发育G1谱系和亚谱系相关的多个特定氨基酸替换。值得注意的是，至少有三种不同的轮状病毒G1分离株在日本儿科人群中共同传播。
A total of 603 fecal specimens collected from July 2005 to June 2006 from children with acute gastroenteritis, encompassing five different localities in Japan, were screened for group A rotavirus by reverse transcription-PCR. It was found that 117 fecal specimens (19.4%) were positive for group A rotavirus. Rotavirus infection was detected continuously from November to June, with the highest prevalence in April. The G (VP7 genotypes) and P (VP4 genotypes) types were further investigated. The predominant genotype was G1P[8] (70.1%), followed by G3P[8] (17.90/c), G9P[8] (6.8%), and G2P[4] (2.6%). A number of unusual G1P[4] combinations were also detected during this study period. A novel nomenclature for G1 is proposed, in which worldwide rotavirus G1 strains are classified into 11 lineages with 17 sublineages. A wide range of amino acid substitutions (up to 34) in VP7 that are specific for G1 lineages and sublineages were identified. Interestingly, only short amino acid motifs located at positions 29 to 75 and 211 to 213 of VP7 defined correctly the phylogenetic G1 lineages and sublineages. Examination of the deduced sequences of antigenic regions of VP7 also revealed multiple particular amino acid substitutions that correlated with the phylogenetic G1 lineages and sublineages. Of note, at least three distinct clusters of rotavirus G1 isolates were cocirculating in the Japanese pediatric population studied.