Sequence analysis of recent H7 avian influenza viruses associated with three different outbreaks in commercial poultry in the United States
Sequence analysis of recent H7 avian influenza viruses associated with three different outbreaks in commercial poultry in the United States
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DOI：
10.1128/jvi.77.24.13399-13402.2003
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发表时间：
2003-12-01
影响因子：
5.4
通讯作者：
Suarez, DL
中科院分区：
文献类型：
--
作者：
Spackman, E;Senne, DA;Suarez, DL
对1994-2002年从美国东北部活禽市场和三起商品禽类暴发中分离到的H7禽流感病毒的血凝素(HA)和神经氨酸酶(NA)基因进行了特征分析。RA和NA基因的系统发育分析表明，商品禽类的分离株与LBM中传播的病毒密切相关。此外，自1994年以来，在该AIV谱系中出现了两个明显的遗传特征：NA蛋白茎区17个氨基酸的缺失和HA1蛋白8个氨基酸的缺失，HA1蛋白可能位于部分受体结合部位。此外，对HA裂解位点氨基酸序列(鸡和火鸡致病性的标志)的分析表明，裂解位点序列的进展符合高致病性AIV的分子标准。
The hemagglutinin (HA) and neuraminidase (NA) genes of H7 avian influenza virus (AIV) isolated between 1994 and 2002 from live-bird markets (LBMs) in the northeastern United States and from three outbreaks in commercial poultry have been characterized. Phylogenetic analysis of the RA and NA genes demonstrates that the isolates from commercial poultry were closely related to the viruses circulating in the LBMs. Also, since 1994, two distinguishing genetic features have appeared in this AIV lineage: a deletion of 17 amino acids in the NA protein stalk region and a deletion of 8 amino acids in the HA1 protein which is putatively in part of the receptor binding site. Furthermore, analysis of the HA cleavage site amino acid sequence, a marker for pathogenicity in chickens and turkeys, shows a progression toward a cleavage site sequence that fulfills the molecular criteria for highly pathogenic AIV.